Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Tlr2 and Tlr4 in Cutaneous Leishmaniasis Caused by Leishmania Major Publisher Pubmed



Tolouei S1 ; Hejazi SH1 ; Ghaedi K2 ; Khamesipour A3 ; Hasheminia SJ4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran
  3. 3. Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Scandinavian Journal of Immunology Published:2013


Abstract

Cutaneous leishmaniasis (CL) is a self-healing skin disease which rarely for unknown reason(s) the lesion develops to a non-healing form. It seems that the initial contact of Leishmania parasites with the host innate immune system is an important step in the outcome of the disease. Recent studies suggested that toll-like receptors (TLRs) play a role in Leishmania recognition. In this study, the level of TLR2 and TLR4 was checked in patients with healing form of lesion and compared with that of patients with non-healing form of lesion caused by Leishmania major. Gene expression of TLR2 and TLR4 in peripheral blood-derived macrophages, before and after stimulation with live L. major promastigotes, was evaluated using quantitative real-time reverse transcription PCR and flow cytometry. The results showed that the mean relative gene expression and difference membrane expression of TLR2 in macrophages of patients with healing form of lesion were significantly higher than patients with non-healing form of lesion (P < 0.0001 and P = 0.0034), respectively, and the mean relative gene expression and difference in protein expression of TLR4 in macrophages of patients with healing form of lesion were significantly higher than that of patients with non-healing form of lesion (P = 0.021 and P = 0.002), respectively. The data suggested a possible role for TLR2 and TLR4 in the outcome of CL lesion. Further studies are needed to understand more about the detail role of the immune factors in leishmaniasis. © 2013 John Wiley & Sons Ltd.
Related Docs
View other Related Docs
1. Il-23 and Il-27 Levels in Macrophages Collected From Peripheral Blood of Patients With Healing Vs Non-Healing Form of Cutaneous Leishmaniasis, Iranian Journal of Parasitology (2012)
2. Study of Il-12 Agonists in Macrophages From Patients With Healing and Non-Healing Forms of Cutaneous Leishmaniasis, Journal of Isfahan Medical School (2013)
3. Interleukin-10 and Transforming Growth Factor-Β in Early and Late Lesions of Patients With Leishmania Major Induced Cutaneous Leishmaniasis, Iranian Journal of Parasitology (2012)
4. Interleukin-10 and Transforming Growth Factor-Β in Early and Late Lesions of Patients With Leishmania Major Induced Cutaneous Leishmaniasis, Iranian Journal of Parasitology (2012)
5. Comparison of Immune Regulatory Factors in Acute and Chronic Lesions of Cutaneous Leishmaniasis Due to Leishmania Major, Journal of Research in Medical Sciences (2014)
6. Identification of Leishmania Isolates From Healing and Nonhealing Cutaneous Leishmaniasis Patients Using Internal Transcribed Spacer Region Pcr, Jundishapur Journal of Microbiology (2014)
7. Regulatory T-Cell Profile in Early and Late Lesions of Cutaneous Leishmaniasis Due to Leishmania Major, Journal of Isfahan Medical School (2014)
8. Evaluation of Il-12Rb1, Il-12B, Cxcr-3 and Il-17A Expression in Cases Affected by a Non-Healing Form of Cutaneous Leishmaniasis: An Observational Study Design, BMJ Open (2017)
Experts (# of related papers)
View all Related Experts
Seyed Hossein Hejazi (47)
Hossein Khanahmad (8)
Other Related Docs
9. Regulatory T-Cell Profile in Early and Late Lesions of Cutaneous Leishmaniasis Due to Leishmania Major, Journal of Research in Medical Sciences (2012)
10. Vaccination in Leishmaniasis: A Review Article, Iranian Biomedical Journal (2022)
11. Serum Monocyte Chemotactic Protein-1 and Nitric Oxide in Cutaneous Leishmaniasis Patients Before and After Treatment With Glucantime, Journal of Isfahan Medical School (2012)
12. The Role of Liposomal Cpg Odn on the Course of L. Major Infection in Balb/C Mice, Iranian Journal of Parasitology (2010)
13. The Comparison of the Ifn-ɤ, Tnf-Α and Il-10 Cytokines in Healing and Non-Healing Cutaneous Leishmaniasis, Iranian Journal of Parasitology (2021)
14. Serum Monocyte Chemotactic Protein-1 and Nitric Oxide in Cutaneous Leishmaniasis Patients Before and After Treatment With Glucantime, Journal of Isfahan Medical School (2012)
15. Comparison of Pro-Inflammatory Cytokines of Non-Healing and Healing Cutaneous Leishmaniasis, Scandinavian Journal of Immunology (2017)
16. Exposure to Leishmania Major Modulates the Proportion of Cd4+ T Cells Without Affecting Cellular Immune Responses, Microbiology and Immunology (2007)
17. Development of Liposomes Loaded With Anti-Leishmanial Drugs for the Treatment of Cutaneous Leishmaniasis, Journal of Liposome Research (2013)
18. Leishmanization: Use of an Old Method for Evaluation of Candidate Vaccines Against Leishmaniasis, Vaccine (2005)
19. Comparison of Protein Profile of Killed and Autoclaved Leishmania Major Using Polyacrylamide Gel Electrophoresis, Journal of Isfahan Medical School (2013)
20. Cutaneous Leishmaniasis Reinfection: Skin Disease and Leishmaniasis Research Center, Isfahan, Iran, Journal of Isfahan Medical School (2015)
21. Leishmanicidal Activity of Films Containing Paromomycin and Gentamicin Sulfate Both in Vitro and in Vivo, Iranian Journal of Parasitology (2011)
22. Immune Response Measured in Human Volunteers Vaccinated With Autoclaved Leishmania Major Vaccine Mixed With Low Dose of Bcg, Clinical and Experimental Immunology (2003)
23. Combinatorial Delivery of Antigen and Tlr Agonists Via Plga Nanoparticles Modulates Leishmania Major-Infected-Macrophages Activation, Biomedicine and Pharmacotherapy (2021)
24. Pcr-Rflp Diagnosis and Characterization of Leishmania Species Causing Human Cutaneous Leishmaniasis and Evaluation of Treatment Times With Glucantime in These Patients, Tropical Biomedicine (2016)
25. A Lentiviral Vaccine Expressing Kmp11-Haspb Fusion Protein Increases Immune Response to Leishmania Major in Balb/C, Parasitology Research (2018)
26. Therapeutic Effect of Topical Tamoxifen in Murine Cutaneous Leishmaniasis Caused by Leishmania Major, Antimicrobial Agents and Chemotherapy (2024)
27. A Randomised, Double-Blind, Controlled Trial of a Killed L. Major Vaccine Plus Bcg Against Zoonotic Cutaneous Leishmaniasis in Iran, Vaccine (1999)
28. Nramp1 Gene Polymorphisms and Cutaneous Leishmaniasis: An Evaluation on Host Susceptibility and Treatment Outcome, Journal of Vector Borne Diseases (2016)
29. Comparative Molecular Epidemiology of Leishmania Major and Leishmania Tropica by Pcr-Rflp Technique in Hyper Endemic Cities of Isfahan and Bam, Iran, Case Reports and Clinical Practice Review (2010)
30. Bacterial Superinfection in Zoonotic Cutaneous Leishmaniasis, Medical Science Monitor (2012)
31. The Efficacy of 5% Trichloroacetic Acid Cream in the Treatment of Cutaneous Leishmaniasis Lesions, Journal of Dermatological Treatment (2012)
32. Molecular Identification and Phylogenetic Classification of Leishmania Spp. Isolated From Human Cutaneous Leishmaniasis in Iran: A Cross-Sectional Study, Iranian Journal of Parasitology (2018)
33. The Study of the Inhibitory Effects of Aqueous and Ethanolic Extracts of Ziziphora Tenuior L. on the Proliferation of Leishmania Major Amastigotes, Iranian Journal of Dermatology (2018)
34. The Skin Side Effects of Topical Glucantime in Patients With Cutaneous Leishmaniasis, Journal of Isfahan Medical School (2015)
35. Effect of Adjunctive Topical Liposomal Azithromycin on Systemic Azithromycin on Old World Cutaneous Leishmaniasis: A Pilot Clinical Study, Iranian Journal of Pharmaceutical Research (2021)
36. Effect of Topical Honey Application Along With Intralesional Injection of Glucantime in the Treatment of Cutaneous Leishmaniasis, BMC Complementary and Alternative Medicine (2007)
37. Locked Nucleic Acid -Anti- Let-7A Induces Apoptosis and Necrosis in Macrophages Infected With Leishmania Major, Microbial Pathogenesis (2018)
38. In Vitro Study of the Leishmanicidal Activity of Perovskia Abrotanoides Terpenoid-Rich Fractions Against Leishmania Major (Mrho/Ir/75/Er), Advanced Biomedical Research (2023)
39. Study of Therapeutic Effect of Different Concentrations of Imatinib on Balb/C Model of Cutaneous Leishmaniasis, AIMS Microbiology (2020)
40. Evaluation of Leishmanin Skin Test Reaction in Different Variants of Cutaneous Leishmaniasis, Indian Journal of Dermatology (2013)
41. Comparative Effect of Topical Trichloroacetic Acid and Intralesional Meglumine Antimoniate in the Treatment of Acute Cutaneous Leishmaniasis, International Journal of Pharmacology (2006)
42. Comparative Molecular Epidemiology of Leishmania Major and Leishmania Tropica by Pcr-Rflp Technique in Hyper Endemic Cities of Isfahan and Bam, Iran, Medical Science Monitor (2010)
43. Anti-Leishmaniasis Effect of Staphylococcus Aureus Protein a on the Size of the Lesion and Parasitic Load, Advanced Biomedical Research (2023)
44. Evaluation of Leishmanin Skin Test and Its Relationship With the Clinical Form and Duration of Cutaneous Leishmaniasis, Dermatology Online Journal (2006)
45. Isolation of Bacteria Causing Secondary Bacterial Infection in the Lesions of Cutaneous Leishmaniasis, Indian Journal of Dermatology (2008)
46. Clinical Picture of Cutaneous Leishmaniasis in Isfahan, Iran, International Journal of Dermatology (1994)
47. The Effect of Medium in Properties and Activity of Acid Phosphatase (Acp) in the Infective Form of Leishmania Major Promastigotes, Journal of Isfahan Medical School (2014)
48. Toll-Like Receptors and Diabetic Nephropathy: A Review of Recent Advances, Turkish Journal of Immunology (2024)
49. Evaluation of the Anti-Leishmanial Effect of Recombinant Clostridium Α-Toxin, Infection and Drug Resistance (2020)
50. Effect of Acupuncture Treatment on Level of Serum Interferon (Ifn)-Γ in Balb/C Model of Leishmania Major Infection, Journal of Isfahan Medical School (2013)