Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Silymarin Suppress Cd4+ T Cell Activation and Proliferation: Effects on Nf-Κb Activity and Il-2 Production Publisher Pubmed



Gharagozloo M1 ; Velardi E2 ; Bruscoli S2 ; Agostini M2 ; Di Sante M2 ; Donato V2 ; Amirghofran Z3 ; Riccardi C2
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Clinical and Experimental Medicine, Section of Pharmacology, Perugia University Medical School, Perugia, Italy
  3. 3. Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

Source: Pharmacological Research Published:2010


Abstract

Silymarin, a mixture of bioactive flavonolignans isolated from Silybum marianum, exhibits anti-carcinogenic, anti-inflammatory and cytoprotective effects. In this study, the in vitro immunomodulatory activity of silymarin was investigated using CD4+ splenocytes from C57/Bl6 mice. Proliferation assay revealed that silymarin, at 50. μM concentration, significantly inhibited CD4+ cells proliferation. ELISA analyses indicated that silymarin significantly inhibited IL-2 and IFN-γ production. Immunofluorescence staining performed on the mouse hybridoma T cell line (3DO) revealed a block of nuclear translocation of transcription factor κB (NF-κB), which is known to be responsible for IL-2 transcriptional activation. Moreover, silymarin inhibited p65/NF-κB phosphorylation in CD4+ T cell. These results suggest that silymarin is able to inhibit T cell activation and proliferation, notably acting on pathways of NF-κB activation/translocation. © 2010 Elsevier Ltd.
Other Related Docs