Production and Characterization of Monoclonal Antibodies Against Brucella Abortus S (99) Surface Antigens

Production and Characterization of Monoclonal Antibodies Against Brucella Abortus S (99) Surface Antigens

Farshad S¹ ; Mehrabanpour MJ² ; Namavari MM² ; Hosseini SMH² ; Tavakkoli A¹ ; Ghaderi A³

Show Affiliations

1. Dept. of Microbiology, Medical School, Isfahan Univ. of Medical Sciences, Isfahan, Iran
2. Razi Serum/Vaccine Res. Institute, Shiraz, Iran
3. Dept. of Immunology, Medical School, Shiraz Univ. of Medical Sciences, Shiraz, Iran

Source: Iranian Biomedical Journal Published:2002

Abstract

By immunizing mice with killed whole bacterial cells of Brucella abortus S (99), a panel of six hybridomas producing monoclonal antibodies (mAb) specific for the surface antigens of this bacterium were produced. ELISA was used to screen the hybridoma supernatants. Immunoblots of the cell extract indicated that three mAb were specific for S-LPS (Ba-1, Ba-2, Ba-3) and three others were reactive with major outer membrane proteins (OMP) (Ba-4, Ba-5, Ba-6). The OMP recognized by these antibodies were the proteins with molecular masses of 25-27 kDa (Ba-4, Ba-5) and 36-38 kDa (Ba-6). None of the four mAb including Ba-3, Ba-4, Ba-5 and Ba-6 cross reacted with any other bacteria close to Brucella abortus, but Ba-1 and Ba-2 cross reacted with B. melitensis 16M and B. suis. By using cell extract and killed whole cell Ag in ELISA, it was indicated that all mAb except Ba-6 have better reactivity with cell extract Ag, but Ba-6 mAb reacted with killed whole cell Ag better than cell extract Ag.

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Style	Citing Format
MLA	Farshad S, et al.. "Production and Characterization of Monoclonal Antibodies Against Brucella Abortus S (99) Surface Antigens." Iranian Biomedical Journal, vol. 6, no. 1, 2002, pp. 7-12.
APA	Farshad S, Mehrabanpour MJ, Namavari MM, Hosseini SMH, Tavakkoli A, Ghaderi A (2002). Production and Characterization of Monoclonal Antibodies Against Brucella Abortus S (99) Surface Antigens. Iranian Biomedical Journal, 6(1), 7-12.
Chicago	Farshad S, Mehrabanpour MJ, Namavari MM, Hosseini SMH, Tavakkoli A, Ghaderi A. "Production and Characterization of Monoclonal Antibodies Against Brucella Abortus S (99) Surface Antigens." Iranian Biomedical Journal 6, no. 1 (2002): 7-12.
Harvard	Farshad S et al. (2002) 'Production and Characterization of Monoclonal Antibodies Against Brucella Abortus S (99) Surface Antigens', Iranian Biomedical Journal, 6(1), pp. 7-12.
Vancouver	Farshad S, Mehrabanpour MJ, Namavari MM, Hosseini SMH, Tavakkoli A, Ghaderi A. Production and Characterization of Monoclonal Antibodies Against Brucella Abortus S (99) Surface Antigens. Iranian Biomedical Journal. 2002;6(1):7-12.
BibTex	@article{ author = {Farshad S and Mehrabanpour MJ and Namavari MM and Hosseini SMH and Tavakkoli A and Ghaderi A}, title = {Production and Characterization of Monoclonal Antibodies Against Brucella Abortus S (99) Surface Antigens}, journal = {Iranian Biomedical Journal}, volume = {6}, number = {1}, pages = {7-12}, year = {2002} }
RIS	TY - JOUR AU - Farshad S AU - Mehrabanpour MJ AU - Namavari MM AU - Hosseini SMH AU - Tavakkoli A AU - Ghaderi A TI - Production and Characterization of Monoclonal Antibodies Against Brucella Abortus S (99) Surface Antigens JO - Iranian Biomedical Journal VL - 6 IS - 1 SP - 7 EP - 12 PY - 2002 ER -

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