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Evaluation of Apoptosis Induction Using Parp Cleavage on Gastric Adenocarcinoma and Fibroblast Cell Lines by Different Strains of Helicobacter Pylori Publisher Pubmed



Mojtahedi A1 ; Salehi R2 ; Navabakbar F3 ; Tamizifar H3 ; Tavakkoli H4 ; Duronio V5
Authors
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Authors Affiliations
  1. 1. Department of Medical Microbiology, Faculty of Medicine, Guilan University Complex, Rasht, 3477, Rasht-Tehran Road, Iran
  2. 2. Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81744-176, Iran
  3. 3. Department of Medical Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81744-176, Iran
  4. 4. Internal Medicine (Gastroenterology), School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81744-176, Iran
  5. 5. Department of Experimental Medicine, School of Medicine, British Columbia University, Vancouver, BC, Canada

Source: Pakistan Journal of Biological Sciences Published:2007


Abstract

Helicobacter pylori is one of the most common pathogens affecting humans and is the major environmental factor in the development of gastric cancer increasing from 4 to 6 folds the risk of its development. Variations in cancer risk among H. pylori infected individuals may correlate to difference in H. pylori strains, variable host characteristics and specific interactions between host and microbial determinants. To determine the effect of different strains of H. pylori on cellular apoptosis this study was designed an in vitro model using AGS and HEF cell lines. After specified time intervals total cell proteins was extracted and subjected to SDS-PAGE and immunoblotting using anti poly ADP-ribose polymerase (PARP) antibody. Decrease in densitometric value of PARP was indicative of higher level of apoptosis. The ability of apoptosis induction in AGS and HEF cell lines by wild type (cagA+/vacA+), cagA-/vacA+, vacA-/cagA+ and double negative (cagA-/vacA_) strains were significantly different. The assessed apoptosis in AGS cell line co-cultured with wild type strain was 3.22±0.2 in 24 h, 2.8±0.1 in 48 and 2.1±0.09 in 72 h of incubation time. Similar assessment with cagA-/vacA + strains in AGS cells was 4.17±1.49 in 24 h, 3.32±0.45 in 48 h and 2.32±0.61 in 72 h incubation. A variation in apoptotic potential between the H. pylori strains on two cells (AGS and HEF) was observed. Based on present results, it is concluded that H. pylori strains as well as target cell types are important in pathogenesis and induction of apoptosis during a specified time interval. © 2007 Asian Network for Scientific Information.
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