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The Frequency of Grasp Gene's Promoter Methylation in Colorectal Cancer Tissue Compared With Healthy Surrounding Tissue Publisher



Khodadoostan M1, 6 ; Ataiekhorasghani M2 ; Saberi F3, 7 ; Jafaripozve N4 ; Shavakhi A5 ; Shavakhi A5 ; Salehi R3, 7
Authors
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Authors Affiliations
  1. 1. Department of Gastroenterology and Hepatology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Gastroenterology and Hepatology, School of Medicine, Khomein University of Medical Sciences, Khomein, Iran
  3. 3. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Department of Maxillofacial Radiology, School of Dentistry, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
  5. 5. Department of Internal Medicine, School of Medicine, Shakrekord University of Medical Sciences, Shakrekord, Iran
  6. 6. Department of Gastroenterology and Hepatology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  7. 7. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Journal of Isfahan Medical School Published:2021


Abstract

Background: The colorectal cancer (CRC) is one of the most common cancers. There are several invasive and noninvasive methods for detecting CRC. Diagnosis based on the molecular markers in early stages seems to be a good choice. The aim of present study was evaluating the methylation in GRASP gen promoter in colorectal cancer tissue compared with healthy surrounding tissue. Methods: This cross-sectional study was performed on 50 patients suffered from CRC during 2016-2019. The DNA was extracted from 50 Formalin-Fixed Paraffin-Embedded (FFPE) samples of CRC tissues in early stages (I and II), and normal surrounding tissues. In paraffin method, methylation-quantification of endonuclease-resistant DNA was calculated, and their frequency were compared in both groups. Findings: GRASP gene's promoter was highly methylated in patients with CRC in early stages compared to their normal margins. GRASP gen promoter methylation was 70.47% (P < 0.00001) in CRC tissue compared with 38.08% in normal margin (P < 0.0001). Conclusion: The results showed that frequency of Grasp gen's promoter methylation in colorectal cancerous tissue was significantly more than normal surrounding tissue. © 2021 Isfahan University of Medical Sciences(IUMS). All rights reserved.
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