Thyroid Peroxidase in Human Endometrium and Placenta: A Potential Target for Anti-Tpo Antibodies Publisher Pubmed

Summary: TPO expressed in endometrium & placenta, potentially linked to abortion & infertility in thyroid autoimmunity. #ThyroidAutoimmunity #Pregnancy

Rahnama R¹ ; Mahmoudi AR² ; Kazemnejad S³ ; Salehi M⁴ ; Ghahiri A⁵ ; Soltanghoraee H⁶ ; Vafaei S⁷ ; Rezaei A¹ ; Zarnani AH^{7, 8, 9}
Source: Clinical and Experimental Medicine Published:2021

Abstract

Autoimmune thyroid disease is the most common endocrine disorder during pregnancy. Thyroid autoantibodies (TAs) have been suggested to serve a role in implantation failure and spontaneous abortion. Until now, there are no data on the potential interaction of TAs with human reproductive organs. Here, we set out for the first time to test this hypothesis by studying the expression of thyroid peroxidase (TPO) at gene and protein level in human reproductive organs. Endometrial samples were taken from normal women, and placenta tissues were collected after full-term caesarian section. Expression of TPO messenger RNA (mRNA) was investigated by qRT-PCR. In addition, polyclonal anti-TPO antibodies were produced and the expression of TPO protein in mentioned tissues was evaluated by immunohistochemistry and Western blot analysis. The reactivity of anti-TPO antibody in human embryos was evaluated by immunofluorescent staining. For the first time, our study showed that TPO is expressed at gene and protein levels in endometrium and placenta. TPO expression was mainly localized to glandular and luminal epithelial cells in the endometrium. In placenta, the syncytiotrophoblasts and invasive trophoblast cells were the main cell types that expressed TPO protein. Specific band of approximately 110 kDa was observed in all endometrial and placental tissues by Western blot analysis. However, no expression of TPO protein was observed in human embryo. TPO expression in endometrium and placenta may explain higher frequency of abortion and infertility in patients with thyroid autoimmunity. © 2020, Springer Nature Switzerland AG.