Mucosal and Systemic Immune Responses Elicited by Recombinant Lactococcus Lactis Expressing a Fusion Protein Composed of Pertussis Toxin and Filamentous Hemagglutinin From Bordetella Pertussis Publisher Pubmed



Torkashvand A¹ ; Bahrami F² ; Adib M¹ ; Ajdary S² Show Affiliations
Source: Microbial Pathogenesis Published:2018

Abstract

We constructed a food-grade expression system harboring a F1S1 fusion protein of Bordetella pertussis to be produced in Lactococcus lactis NZ3900 as a new oral vaccine model against whooping cough, caused by B. pertussis. F1S1 was composed of N-terminally truncated S1 subunit of pertussis toxin and type I immunodominant domain of filamentous hemagglutinin which are both known as protective immunogens against pertussis. The recombinant L. lactis was administered via oral or intranasal routes to BALB/c mice and the related specific systemic and mucosal immune responses were then evaluated. The results indicated significantly higher levels of specific IgA in the lung extracts and IgG in sera of mucosally-immunized mice, compared to their controls. It was revealed that higher levels of IgG2a, compared to IgG1, were produced in all mucosally-immunized mice. Moreover, immunized mice developed Th1 responses with high levels of IFN-γ production by the spleen cells. These findings provide evidence for L. lactis to be used as a suitable vehicle for expression and delivery of F1S1 fusion protein to mucosa and induction of appropriate systemic and mucosal immune responses against pertussis. © 2018 Elsevier Ltd