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Cytotoxicity Evaluation of 63S Bioactive Glass and Bone-Derived Hydroxyapatite Particles Using Human Bone-Marrow Stem Cells Publisher Pubmed



Doostmohammadi A1, 2 ; Monshia A1 ; Salehic R3 ; Fathia MH1 ; Seyedjafarid E4 ; Shafieee A5 ; Soleimanif M6
Authors
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Authors Affiliations
  1. 1. Biomaterials Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran
  2. 2. Isfahan University of Medical Science, Isfahan, 81746-73461, Iran
  3. 3. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sci ences, Isfahan, 81746-73461, Iran
  4. 4. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran
  5. 5. Stem cell Biology department, Stem cell technology research center, Tehran, Iran
  6. 6. Department of hematology, Faculty of Medical Sciences, Tarbiat modares University, Tehran, Iran

Source: Biomedical Papers Published:2011


Abstract

Background. In recent years, bioceramics have been favored by biomaterials scientists and researchers. Due to their special and distinctive features, bioactive glass and hydroxyapatite possess a higher place among different types of bioceramics. Method. In this study, the effect of 63S bioactive glass and bone-derived hydroxyapatite particles on the proliferation of human bone-marrow stem cells (hMSCs) was investigated. Bioactive glass particles were made via sol-gel method and hydroxyapatite was obtained from bovine bone. The particle size and morphology were investigated by scanning electron microscope (SEM). Then the in vitro cytotoxicity of particles was evaluated using MTT assay. SEM showed that bioactive glass particles were in the nanoscale range and had tendency towards agglomeration. It was also confirmed that the hydroxyapatite particles were agglomerations of crystals cca 50-500 nm across. Results. The results of MTT assay confirmed the viability and proliferation of hMSCs in contact with bioactive glass and bone-derived HA particles. The fabricated particles in combination with stem cells were shown to hold promising potential for further applications in tissue engineering and regenerative medicine. © A. Doostmohammadi, A. Monshi, R. Salehi, M. H. Fathi, E. Seyedjafari, A. Shafiee, M. Soleimani.
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