Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share By
A New Spectrophotometric Method for Direct Determination of Iron (Iii) in Serum



Jafariandehkordi A1 ; Saghaie L2 ; Movahedi N1
Authors

Source: Daru Published:2008

Abstract

Bakground and purpose of the study: Hydroxypyridin-4-ones, a group of iron chelators have shown promise as potential compounds for the treatment of iron overload by the oral route. Their selectivity and high complex formation constant with iron makes them good candidates for iron determination. In this study the use of N-ethyl-2-methyl-3-hydroxypyridin-4-on (EMHP), a strong and selective ferric chelator, as a new ligand for measurement of ?molar concentrations of iron in aqueous solutions and biological fluids was investigated. This measurement is based on the color reaction of Fe3+ with EMHP. Methods: After mixing serum sample and reagent, and incubating at room temperature, the absorbance of the resulting complex was measured at λmax. The effect of analytical variables, such as the amount and the kind of the reagents, pH, ratio of EMHP/Fe (III) and presence of other ions in determination of iron were studied. Results: The results showed that the optimum wavelength for the measurement was 456 nm. Formation of the complex was completed in less than 20 min and it was stable up to 24 hrs. Molar ratio of 6-10 EMHP/Fe (III) and pH = 5 were the optimum conditions for complex formation and determination of Fe (III). The detection limit was 2.5×10-6 M of Fe (III) in serum or plasma. Ions commonly associated with iron did not interfere in the present method. Conclusion: This method which is simple and reproducible was found sensitive for determination of Fe (III) in several real samples at micromolar levels.
Other Related Docs
4. Hydroxypyridinone Derivatives: Synthesis and Cytotoxic Evaluation, Journal of Reports in Pharmaceutical Sciences (2013)
9. Evaluation of Iron Status by Serum Ferritin Level in Iranian Carriers of Beta Thalassemia Minor, International Journal for Vitamin and Nutrition Research (2008)
10. The Effect of Chromium on Parameters Related to Iron Metabolism, Biological Trace Element Research (1992)
14. Investigation of Intestinal Absorption of Pyridinones in Rat, Iranian Journal of Pharmaceutical Research (2004)