Ameliorative Effects of Omega-Lycotoxin-Gsp2671e Purified From the Spider Venom of Lycosa Praegrandis on Memory Deficits of Glutamate-Induced Excitotoxicity Rat Model Publisher



Keimasi M¹ ; Salehifard K² ; Shahidi M¹ ; Esmaeili F¹ ; Mirshah Jafar Esfahani N¹ ; Beheshti S¹ ; Amirsadri M³ ; Naseri F⁴ ; Keimasi M¹ ; Ghorbani N¹ ; Mofid MR⁵ ; Moradmand M¹ Show Affiliations
Source: Frontiers in Pharmacology Published:2022

Abstract

Memory impairment is one of the main complications of Alzheimer’s disease (AD). This condition can be induced by hyper-stimulation of N-Methyl-D-aspartate receptors (NMDARs) of glutamate in the hippocampus, which ends up to pyramidal neurons determination. The release of neurotransmitters relies on voltage-gated calcium channels (VGCCs) such as P/Q-types. Omega-lycotoxin-Gsp2671e (OLG1e) is a P/Q-type VGCC modulator with high affinity and selectivity. This bio-active small protein was purified and identified from the Lycosa praegrandis venom. The effect of this state-dependent low molecular weight P/Q-type calcium modulator on rats was investigated via glutamate-induced excitotoxicity by N-Methyl-D-aspartate. Also, Electrophysiological amplitude of field excitatory postsynaptic potentials (fEPSPs) in the input–output and Long-term potentiation (LTP) curves were recorded in mossy fiber and the amount of synaptophysin (SYN), synaptosomal-associated protein, 25 kDa (SNAP-25), and synaptotagmin 1(SYT1) genes expression were measured using Real-time PCR technique for synaptic quantification. The outcomes of the current study suggest that OLG1e as a P/Q-type VGCC modulator has an ameliorative effect on excitotoxicity-induced memory defects and prevents the impairment of pyramidal neurons in the rat hippocampus. Copyright © 2022 Keimasi, Salehifard, Shahidi, Esmaeili, Mirshah Jafar Esfahani, Beheshti, Amirsadri, Naseri, Keimasi, Ghorbani, Mofid and Moradmand.