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Comparative Genetic Defence Analysis Using Microsatellite Markers and Anatomical Resistance of Potato Cultivars to Early Blight Publisher



Alizadehmoghaddam G1 ; Nasresfahani M2 ; Nasresfahani A3 ; Sedaghatfar E4 ; Rahanandeh H5 ; Yazdi MJ6
Authors
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Authors Affiliations
  1. 1. Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, 84517-31167, Iran
  2. 2. Department of Plant Protection Research, Isfahan Agricultural and Natural Resources Research and Education Center, AREEO, Isfahan, Iran
  3. 3. Department of Mycology and Parasitology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  4. 4. Plant Pathology Department, Islamic Azad University, Arak Branch, Arak, Iran
  5. 5. Department of Plant Protection, Islamic Azad University, Rasht Branch, Rasht, Iran
  6. 6. Department of Agronomy and Plant Breeding, College of Agriculture, Ardestan Branch, Islamic Azad University, Ardestan, Iran

Source: Physiological and Molecular Plant Pathology Published:2024


Abstract

Early blight (EB) disease is one of the most devastating threats of potato production. Alternaria alternata and A. solani are the main causative species of the EB disease globally. Thus, in this study, we investigated the response of a diverse collection of ten potato cultivars to EB at pre and post-flowering stages to both species, and their colony growth rates, as well as leaf cuticular wax thickness associated with EB resistance and biomass parameters. We also analysed genetic diversity of the related cultivars using eleven simple sequence repeat (SSR) markers accordingly. Based on the mean disease severity percentage (DSP), A. alternata was more pronounced than A. solani on the related potato cultivars at both growth stages, significantly. The highest DSP to A. alternata was on “Burren” (80 %) and “White Desiree” (77 %), and the lowest one on “Agria” (7 %). Whereas, the highest DSP to A. solani was on “Burren” (52 %), and the lowest one on “Agria” (17 %). The wax thickness of the adaxial cuticle on the fifth leaf varied from 0.9 μ to 1.5 μ and for abaxial cuticle varied from 0.6 μ to 1.4 μ. The highest epicuticular wax layer was of partially resistant “Agria” and moderately resistant “Santana” (1.3–1.5 μ), and the lowest one on susceptible “White Desiree”, “Milva” and “Burren” (0.8, 0.9 μ, respectively). Biomass analysis showed lower values of growth parameters in the susceptible cultivars than the resistant ones, significantly. According to primer polymorphism analysis, eleven SSR primers were polymorphic. The selected SSR primers showed significant genetic diversity with the polymorphism information content ranging from 0.1 to 0.5 (mean 0.4), effective multiplex ratio from 0.7 to 12.3 (mean 4.3), marker index from 0.04 to 6 (mean 2), and resolving power from 0.6 to 12.8 (mean 5). Cluster analysis of SSR data revealed considerable genetic diversity between the related potato cultivars, suggesting a significant genetic variation between resistant and susceptible ones. This work may improve our understanding of genetic diversity and wax thickness as markers for identifying resistant potato cultivars to EB disease. © 2024 Elsevier Ltd
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