Analysis of Polymorphism and Expression Profile of Asic1 and Il-6 Genes in Patients With Gastric Cancer Publisher Pubmed



Heydarimehrabadi A¹ ; Tamandani DMK¹ ; Baranzehi T¹ ; Hemati S²
Source: Asian Pacific Journal of Cancer Prevention Published:2018

Abstract

Gastric cancer is one of the most common upper gastrointestinal malignancies. Some Iranian provinces, such as in the northern and northwestern areas, are at a high risk, whereas the central and western provinces are at a medium and the southern regions at low risk. This study was carried out to estimate the impact of the expression patterns of ASIC1 and IL-6 genes and the IL-6rs-174 and ASIC1rs 75624685 polymorphisms in the pathogenesis of gastric cancer. Materials and methods: Tetra-ARMS PCR was employed to analyze the polymorphism status of the ASIC1 and IL-6 genes with 85 paraffin-embedded tissue blocks from cases and 117 normal blood samples as controls. We also investigated mRNA expression levels of these genes in 12 cases and controls using real-time PCR. Results: Our results showed a significant association between expression of ASIC1 and elevated risk of gastric cancer (p < 0.001). © 2018, Asian Pacific Organization for Cancer Prevention.