The Comparison of Diphenhydramine Hcl and Nedocromil Sodium in Prevention of Abdominal Postoperative Adhesion Formation in Rat Models: An Experimental Study Publisher Pubmed



Rasti M¹ ; Parvaresh E¹ ; Tavajoh S¹ ; Talaei M¹ Show Affiliations
Source: International Journal of Surgery Published:2007

Abstract

Background: The purpose of this study was to determine the effects of diphenhydramine HCl and Nedocromil sodium for the prevention of postsurgical adhesion formation in rat model. Methods: Sixty adult female rats were anesthetized by 5 mg/kg ketamine hydrochloride. After opening the abdominal wall, a 2 cm2 peritoneal layer was excised from the left abdominal wall and 10 longitudinal incisions of 2 to 3 cm in length were made on the right parietal peritoneum. The abdominal wall was closed with 4/0 atraumatic continuous nylon sutures. Group I was the control group, group II was given 10 mg/kg diphenhydramine HCl, group III was given 100 mg/kg Nedocromil sodium, and group IV was administered both drugs in the above doses. All the drugs were instilled into the peritoneal cavity after abdominal closure except Nedocromil sodium which was administered in two separate doses 30 min before surgery and just after abdominal closure. Relaparatomy was performed 2 weeks after the initial surgery and abdominal adhesions were scored. Kruskal-Wallis and Mann-Whitney U-test were used for the statistical evaluation. Results: The mean ± S.D. (median) of adhesion scores were 2.5 ± 0.90 (2.0), 1.58 ± 0.99 (1.0), 0.92 ± 0.86 (1.0) and 1.75 ± 0.75 (2.0) in group I, II, III and IV, respectively. There were significant differences between the scores of groups I and II (P = 0.033), groups I and III (P < 0.001), and groups I and IV (P = 0.033). Conclusion: Both diphenhydramine HCl and Nedocromil sodium reduced postoperative abdominal adhesions separately and in combination with each other in our study. Average score of adhesion formation was lowest in the group that was administered Nedocromil sodium. More research is needed in order to discover any positive effect of these drugs as antiadhesive agents in humans. © 2007 Surgical Associates Ltd.