Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Investigation of the Association Between (Ccttt)N Polymorphism in Nos2 Gene and Serum Ige Level in Patients With Vernal Keratoconjunctivitis in Chaharmahal Va Bakhtiari Province Publisher



Aali F1 ; Barjui SP2 ; Aali F1 ; Reiisi S4 ; Chaleshtori MH2 ; Salehi A5
Authors
Show Affiliations
Authors Affiliations
  1. 1. Shahrekord University of Medical Sciences, Shahrekord, Iran
  2. 2. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
  3. 3. Islamic Azad University Shahrekord Branch, Shahrekord, Iran
  4. 4. Department of Genetics, Faculty of Basic Sciences, University of Shahrekord, Shahrekord, Iran
  5. 5. Ophthalmology Department, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Gene Reports Published:2018


Abstract

Introduction: Vernal keratoconjunctivitis (VKC) disease is an immune response of the body caused by environmental antigens leading to the inflammation of the conjunctive tissue. Many factors have been regarded as potential contributing factors in VKC development. NOS2 gene is one of the genetics factors that can be supposed as a potential contributor in the disorder development. In this research, the association between (CCTTT)n polymorphism in NOS2 gene promoter was investigated to find the presumable emolument. In the Iranian populations have not any reported the association of polymorphism CCTTT (n) with the promoter of the NOS2 gene. Methods and materials: The study included 200 individuals (100 patients with VKC and 98 controls). After DNA extraction, PCR was performed following a standard phenol chloroform protocol, and then Genotypes were identified using polyacrylamide gel electrophoresis (PAGE). Serum IgE levels were estimated using Elisa Kit. Interpretation of findings was accomplished using SPSS software after analyzing the quality and quantity criteria associated with the signs and symptoms of the disease. Results: (CCTTT)n polymorphism allele analysis in our study showed 10 alleles in patient group and 11 alleles in control group. The difference of allele (CCTTT) frequency between the patient and control groups was significant (P-value < 0.001). Serum IgE levels in both groups did not show statistically significant differences (P-value = 23%). Conclusion: In this study, we verified the association of (CCTTT)12 and VKC disease. Therefore, the concerned nucleotide change can be employed as an informative tool in determining the disease prognosis and detection of carriers, identification tests and elucidating the mechanism by which VKC develops. © 2018
Related Docs
View other Related Docs
1. Mutation Screening of 3’Utr and Exons 1-2 of Vsx1 Gene by Pcr-Sscp/Ha and Sequencing in Patients With Vernal Keratoconjuctivis (Vkc) in Shahrekord, Journal of Zanjan University of Medical Sciences and Health Services (2015)
2. Comparative Evaluation of Tacrolimus Versus Interferon Alpha-2B Eye Drops in the Treatment of Vernal Keratoconjunctivitis: A Randomized, Double-Masked Study, Cornea (2017)
3. Mutation Analysis of Vsx1 Gene in Vernal Keratoconjunctivitis Patients, Meta Gene (2020)
4. Evaluation of the Frequency of Gene Polymorphism of Interleukin-7 Receptor Subunit Alpha (Il7r-Α) in Atopic Patients and Healthy Controls, Journal of Isfahan Medical School (2016)
5. Association Analysis of T-Cell Immunoglobulin and Mucin 1 Gene 5383-5397 Insertion/Deletion Polymorphism With Asthma and Total Serum Immunoglobulin E Levels in Patients With Asthma and Controls, Journal of Isfahan Medical School (2014)
6. Studying Vsx1 Gene Mutations in Patients With Keratoconus of Chaharmahal and Bakhtiari Province, Iran, Journal of Kerman University of Medical Sciences (2013)
7. Association Analysis of -416 G>C Polymorphism of T-Cell Immunoglobulin and Mucin Domain-1 Gene With Asthma in Iran, International Journal of Immunogenetics (2015)
8. Evaluation of Common Col2a1 Gene Variants in Iranian Patients Suspected With Stickler Syndrome Type I, Journal of Research in Medical Sciences (2025)
Experts (# of related papers)
View all Related Experts
Rasoul Salehi (3)
Ali Salehi (2)
Other Related Docs
9. Study of Vsx1 Mutations in Patients With Keratoconus in Southwest Iran Using Pcr-Single-Strand Conformation Polymorphism/Heteroduplex Analysis and Sequencing Method, Acta Cytologica (2013)
10. The Hhex Rs1111875a/G Gene Polymorphism Is Associated With Susceptibility to Type 2 Diabetes in the Iranian Population, Molecular Biology (2015)
11. The Association Between the C(-1562)T Polymorphism of Type Iv Collagenase Gene and Reduced Age of Onset of Lung Cancer, Journal of Isfahan Medical School (2012)
12. Serum Vitamin D Levels in Patients With Vernal Keratoconjunctivitis: A Systematic Review and Meta-Analysis, Ocular Immunology and Inflammation (2024)