Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Synthesis of Pectin-Deoxycholic Acid Conjugate for Targeted Delivery of Anticancer Drugs in Hepatocellular Carcinoma Publisher Pubmed



Varshosaz J1 ; Sadri F1 ; Rostami M2 ; Mirian M3 ; Taymouri S1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Novel Drug Delivery Systems Research Center, Department of Pharmaceutics, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Medicinal Chemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
  3. 3. Department of Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

Source: International Journal of Biological Macromolecules Published:2019


Abstract

Sorafenib (SF) a chemotherapeutic drug is used in hepatocellular carcinoma (HCC) with vast side effects. The aim of the project ahead was synthesis of SF loaded co-polymeric micelles of pectin-deoxycholic acid (P-DOCA) to target the overexpressed asialoglycoprotein receptors of hepatocytes by pectin. DOCA was modified with ethylenediamine and conjugated to pectin. FT-IR and 1HNMR confirmed the bio-conjugation. Pyrene was used to measure critical micelle concentration (CMC) by fluorimetry technique. P-DOCA micelles were loaded with SF and their particle size, zeta potential, drug loading and release efficiency were measured. MTT assay was used for determining cytotoxicity. The cell cycle arrest was studied by flow cytometry analysis and the cellular uptake was studied using cumarin-6 as the fluorophore agent. The micelles capability in preventing the cells migration was tested by Transwell plates. The CMC of P-DOCA micelles was 10.747 μg/mL. The best formulation obtained from SF to polymer ratio of 1:2. SF loaded micelles showed 30% increased cytotoxicity. The micelles cellular uptake was more than the free drug. Relative migration of HepG2 cells treated with SF loaded micelles was reduced to 6.67% compared to free SF which was 26.67%. The designed micelles are promising for antitumor drug targeting to HCC. © 2019
Related Docs
View other Related Docs
1. In Vitro and in Vivo Evaluation of Novel Dtx-Loaded Multifunctional Heparin-Based Polymeric Micelles Targeting Folate Receptors and Endosomes, Recent Patents on Anti-Cancer Drug Discovery (2020)
2. Polyacrylamide-Punicic Acid Conjugate-Based Micelles for Flutamide Delivery in Pc3 Cells of Prostate Cancer: Synthesis, Characterisation and Cytotoxicity Studies, IET Nanobiotechnology (2020)
3. In Vivo Pharmacokinetics, Biodistribution and Anti-Tumor Effect of Paclitaxel-Loaded Targeted Chitosan-Based Polymeric Micelle, Drug Delivery (2016)
4. Folic Acid Targeted Polymeric Micelles Based on Tocopherol Succinate-Pulluan As an Effective Carrier for Epirubicin: Preparation, Characterization and In-Vitro Cytotoxicity Assessment, Current Drug Delivery (2018)
5. Novel Ph-Triggered Biocompatible Polymeric Micelles Based on Heparin–Α-Tocopherol Conjugate for Intracellular Delivery of Docetaxel in Breast Cancer, Pharmaceutical Development and Technology (2020)
6. Cytotoxic Effects of Targeted Galactosylated Pluronic F127/Pluronicl122 Nano-Sized Micelles Loaded With Doxorubicin in Human Hepatocellular Carcinoma Cell Line, Journal of Isfahan Medical School (2013)
7. Synthesis and in Vitro/In Vivo Characterization of Raloxifene Grafted Poly(Styrene Maleic Acid)-Poly(Amide-Ether-Ester-Imide) Micelles for Targeted Delivery of Docetaxel in G Protein-Coupled Estrogen Receptor Breast Cancer, Anti-Cancer Agents in Medicinal Chemistry (2018)
8. Optimisation of Processing Variables Effective on Self-Assembly of Folate Targeted Synpronic-Based Micelles for Docetaxel Delivery in Melanoma Cells, IET Nanobiotechnology (2015)
Experts (# of related papers)
View all Related Experts
Jaleh Varshosaz (54)
Farshid Hassanzadeh (39)
Other Related Docs
9. Folated Synperonic-Cholesteryl Hemisuccinate Polymeric Micelles for the Targeted Delivery of Docetaxel in Melanoma, BioMed Research International (2015)
10. Pegylated Trimethylchitosan Emulsomes Conjugated to Octreotide for Targeted Delivery of Sorafenib to Hepatocellular Carcinoma Cells of Hepg2, Journal of Liposome Research (2019)
11. Preparation and Characterization of Spray-Dried Inhalable Powders Containing Polymeric Micelles for Pulmonary Delivery of Paclitaxel in Lung Cancer, Journal of Pharmacy and Pharmaceutical Sciences (2018)
12. Development and in Vitro/In Vivo Evaluation of a Novel Targeted Polymeric Micelle for Delivery of Paclitaxel, International Journal of Biological Macromolecules (2015)
13. Synthesis, in Vitro Characterization, and Anti-Tumor Effects of Novel Polystyrene-Poly(Amide-Ether-Ester-Imide) Co-Polymeric Micelles for Delivery of Docetaxel in Breast Cancer in Balb/C Mice, Drug Development and Industrial Pharmacy (2018)
14. Preparation and Evaluation of Targeted Albumin Lipid Nanoparticles With Lactobionic Acid for Targeted Drug Delivery of Sorafenib in Hepatocellular Carcinoma, Journal of Drug Delivery Science and Technology (2022)
15. Self-Assembly Micelles With Lipid Core of Cholesterol for Docetaxel Delivery to B16f10 Melanoma and Hepg2 Cells, Journal of Liposome Research (2015)
16. Synthesis and in Vitro Evaluation of Self-Assembling Biocompatible Heparin-Based Targeting Polymeric Micelles for Delivery of Doxorubicin to Leukemic Cells, Research in Pharmaceutical Sciences (2025)
17. Folate-Targeted Polyacrylamide/Punicic Acid Nanomicelles for Flutamide Delivery in Prostate Cancer: Characterization, in Vitro Biological Evaluation, and Its Dft Study, Recent Patents on Nanotechnology (2020)
18. Effect of Molecular Weight and Molar Ratio of Dextran on Self-Assembly of Dextran Stearate Polymeric Micelles As Nanocarriers for Etoposide, Journal of Nanomaterials (2012)
19. A Novel Mixed Polymeric Micelle for Co-Delivery of Paclitaxel and Retinoic Acid and Overcoming Multidrug Resistance: Synthesis, Characterization, Cytotoxicity, and Pharmacokinetic Evaluation, Drug Development and Industrial Pharmacy (2018)
20. Cytotoxic Effect of Pluronic F127-Poly (Methyl Vinyl Ether-Alt-Maleic Acid) Micelles Loaded With Doxorubicin on Mcf-7 Cells, Journal of Isfahan Medical School (2013)
21. Pharmacokinetics, Organ Toxicity and Antitumor Activity of Docetaxel Loaded in Folate Targeted Cholesterol Based Micelles, Current Drug Delivery (2016)
22. Cytotoxicity and Antitumor Properties of a Marine Compound, Hesa-A, on Cancer Cells, Daru (2003)
23. Chondroitin/Doxorubicin Nanoparticulate Polyelectrolyte Complex for Targeted Delivery to Hepg2 Cells, IET Nanobiotechnology (2017)
24. Novel Tocopherol Succinate-Polyoxomolybdate Bioconjugate As Potential Anti-Cancer Agent, Journal of Inorganic and Organometallic Polymers and Materials (2021)
25. Cytotoxic Activities of Euphorbia Kopetdaghi Against Ovcar-3 and Ej-138 Cell Lines, Journal of HerbMed Pharmacology (2015)
26. Biotin-Encoded Pullulan-Retinoic Acid Engineered Nanomicelles: Preparation, Optimization and in Vitro Cytotoxicity Assessment in Mcf-7 Cells, Indian Journal of Pharmaceutical Sciences (2016)
27. Development of a Rp-Hplc Method for Analysis of Docetaxel in Tumor-Bearing Mice Plasma and Tissues Following Injection of Docetaxel-Loaded Ph Responsive Targeting Polymeric Micelles, Research in Pharmaceutical Sciences (2020)
28. Development and Optimization of Transferrin-Conjugated Nanostructured Lipid Carriers for Brain Delivery of Paclitaxel Using Box–Behnken Design, Pharmaceutical Development and Technology (2017)
29. Magnetic Polyvinyl Caprolactam–Polyvinyl Acetate–Polyethylene Glycol Micelles for Docetaxel Delivery in Breast Cancer: An in Vitro Study on Two Cell Lines of Breast Cancer, Pharmaceutical Development and Technology (2017)
30. An Injectable Thermosensitive Hydrogel/Nanomicelles Composite for Local Chemo-Immunotherapy in Mouse Model of Melanoma, Journal of Biomaterials Applications (2022)
31. Pegylated Multifunctional Ph-Responsive Targeted Polymeric Micelles for Ovarian Cancer Therapy: Synthesis, Characterization and Pharmacokinetic Study, International Journal of Polymeric Materials and Polymeric Biomaterials (2021)
32. Design, Synthesis, and Comparison of Pla-Peg-Pla and Peg-Pla-Peg Copolymers for Curcumin Delivery to Cancer Cells, Polymers (2023)
33. Docetaxel-Loaded Nanostructured Lipid Carriers Functionalized With Trastuzumab (Herceptin) for Her2-Positive Breast Cancer Cells, Journal of Liposome Research (2018)
34. Synthesis and Characterization of Folate-Targeted Dextran/Retinoic Acid Micelles for Doxorubicin Delivery in Acute Leukemia, BioMed Research International (2014)
35. Pharmacokinetics and in Vitro/In Vivo Antitumor Efficacy of Aptamer-Targeted Ecoflex® Nanoparticles for Docetaxel Delivery in Ovarian Cancer, International Journal of Nanomedicine (2018)
36. Synthesis of Biotin-Targeted Chitosan/Poly (Methyl Vinyl Ether- Alt -Maleic Acid) Copolymeric Micelles for Delivery of Doxorubicin, IET Nanobiotechnology (2017)
37. Comparing Different Sterol Containing Solid Lipid Nanoparticles for Targeted Delivery of Quercetin in Hepatocellular Carcinoma, Journal of Liposome Research (2014)
38. Tissue Distribution and Systemic Toxicity Evaluation of Raloxifene Targeted Polymeric Micelles of Poly (Styrene-Maleic Acid)-Poly (Amide- Ether-Ester-Imide)-Poly (Ethylene Glycol) Loaded With Docetaxel in Breast Cancer Bearing Mice, Recent Patents on Anti-Cancer Drug Discovery (2019)
39. Anti-Cancer Activity of Biotin-Polyoxomolybdate Bioconjugate, Eurasian Journal of Medicine and Oncology (2020)
40. Folate Targeted Solid Lipid Nanoparticles of Simvastatin for Enhanced Cytotoxic Effects of Doxorubicin in Chronic Myeloid Leukemia, Current Nanoscience (2012)
41. Co-Delivery of Carboplatin and Doxorubicin Using Zif-8 Coated Chitosan-Poly(N-Isopropyl Acrylamide) Nanoparticles Through a Dual Ph/Thermo Responsive Strategy to Breast Cancer Cells, International Journal of Biological Macromolecules (2024)
42. Co-Delivery of Paclitaxel and Α-Tocopherol Succinate by Novel Chitosan-Based Polymeric Micelles for Improving Micellar Stability and Efficacious Combination Therapy, Drug Development and Industrial Pharmacy (2015)
43. Co-Delivery of Arsenotungstate Polyoxometalate and Curcumin Using Bis-Imidazolium Poly Ionic Liquid Nanocomposites for Boosting Anticancer Effects Against Triple-Negative Breast Cancer Cells, Journal of Inorganic and Organometallic Polymers and Materials (2024)
44. Luteinizing Hormone-Releasing Hormone Targeted Poly(Methyl Vinyl Ether Maleic Acid) Nanoparticles for Doxorubicin Delivery to Mcf-7 Breast Cancer Cells, IET Nanobiotechnology (2016)
45. Monastrol Derivatives: In Silico and in Vitro Cytotoxicity Assessments, Research in Pharmaceutical Sciences (2020)
46. Delivery of Vinblastine-Containing Niosomes Results in Potent in Vitro/In Vivo Cytotoxicity on Tumor Cells, Drug Development and Industrial Pharmacy (2018)
47. Ph-Responsive, Adorned Nanoniosomes for Codelivery of Cisplatin and Epirubicin: Synergistic Treatment of Breast Cancer, ACS Applied Bio Materials (2022)
48. Novel Nanoscale Vanillin Based Covalent Triazine Framework As a Novel Carrier for Sustained Release of Imatinib, Polymers for Advanced Technologies (2023)
49. Inhibitory Effect of Genistein on Plc/Prf5 Hepatocellular Carcinoma Cell Line, International Journal of Preventive Medicine (2015)
50. Enhanced Solubility, Oral Bioavailability and Anti-Osteoporotic Effects of Raloxifene Hcl in Ovariectomized Rats by Igepal Co-890 Nanomicelles, Pharmaceutical Development and Technology (2019)