Quantification of Ergovaline Using Hplc and Mass Spectrometry in Iranian Neotyphodium Infected Tall Fescue

Quantification of Ergovaline Using Hplc and Mass Spectrometry in Iranian Neotyphodium Infected Tall Fescue

Sobhani Najafabadi A¹ ; Mofid MR² ; Mohammadi R¹ ; Moghim S³

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1. Department of Secondary Metabolite, Agricultural Biotechnology Research Institute Central Region of Iran, Isfahan, Iran
2. Department of Biochemistry, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
3. Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Research in Pharmaceutical Sciences Published:2010

Abstract

Ergovaline, the main ergopeptine alkaloid produced in tall fescue (Fescue arundinacea Schreb.) infected with endophyte (Neotyphodium coenophialum Morgan- Jones & Gams), is known to cause tall fescue toxicosis. This study was conducted to examine the presence of fungal endophytes in five populations of tall fescue collected from various regions of Iran. The existence of Neotyphodium mycelia in the tissues of the samples was confirmed by microscopic examination, and the isolation was performed from leaf tissues of the hosts on potato dextrose agar. All isolates were confirmed as the Neotyphodium species by PCR, using specific primers. Mass detection and determination of ergovaline were performed by HPLC at three plant growth stages. Ergovaline was detected in all isolates, with the mean concentrations of 0.24 to 3.48 μg/g dry matter of different populations for the whole three plant growth stages. The differences in ergovaline content between plant populations and sampling time were statistically significant. This is the first report of ergovaline content in endophyte infected Fescue. arundinacea from natural grasslands in Iran.

Style	Citing Format
MLA	Sobhani Najafabadi A, et al.. "Quantification of Ergovaline Using Hplc and Mass Spectrometry in Iranian Neotyphodium Infected Tall Fescue." Research in Pharmaceutical Sciences, vol. 5, no. 2, 2010, pp. 136-143.
APA	Sobhani Najafabadi A, Mofid MR, Mohammadi R, Moghim S (2010). Quantification of Ergovaline Using Hplc and Mass Spectrometry in Iranian Neotyphodium Infected Tall Fescue. Research in Pharmaceutical Sciences, 5(2), 136-143.
Chicago	Sobhani Najafabadi A, Mofid MR, Mohammadi R, Moghim S. "Quantification of Ergovaline Using Hplc and Mass Spectrometry in Iranian Neotyphodium Infected Tall Fescue." Research in Pharmaceutical Sciences 5, no. 2 (2010): 136-143.
Harvard	Sobhani Najafabadi A et al. (2010) 'Quantification of Ergovaline Using Hplc and Mass Spectrometry in Iranian Neotyphodium Infected Tall Fescue', Research in Pharmaceutical Sciences, 5(2), pp. 136-143.
Vancouver	Sobhani Najafabadi A, Mofid MR, Mohammadi R, Moghim S. Quantification of Ergovaline Using Hplc and Mass Spectrometry in Iranian Neotyphodium Infected Tall Fescue. Research in Pharmaceutical Sciences. 2010;5(2):136-143.
BibTex	@article{ author = {Sobhani Najafabadi A and Mofid MR and Mohammadi R and Moghim S}, title = {Quantification of Ergovaline Using Hplc and Mass Spectrometry in Iranian Neotyphodium Infected Tall Fescue}, journal = {Research in Pharmaceutical Sciences}, volume = {5}, number = {2}, pages = {136-143}, year = {2010} }
RIS	TY - JOUR AU - Sobhani Najafabadi A AU - Mofid MR AU - Mohammadi R AU - Moghim S TI - Quantification of Ergovaline Using Hplc and Mass Spectrometry in Iranian Neotyphodium Infected Tall Fescue JO - Research in Pharmaceutical Sciences VL - 5 IS - 2 SP - 136 EP - 143 PY - 2010 ER -

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