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Leishmanization: Use of an Old Method for Evaluation of Candidate Vaccines Against Leishmaniasis Publisher Pubmed



Khamesipour A1 ; Dowlati Y1 ; Asilian A2 ; Hashemifesharki R3 ; Javadi A4 ; Noazin S5 ; Modabber F5, 6
Authors
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Authors Affiliations
  1. 1. Ctr. Res. Train. Skin Dis. Leprosy, Tehran Univ. of Medical Sciences, Tehran 14166, P.O. Box 14155-6383, Iran
  2. 2. Department of Dermatology, Isfahan Univ. of Medical Sciences, Isfahan, Iran
  3. 3. Razi Vacc. and Serum Res. Institute, Hessarak, P.O. Box 11365-1558, Iran
  4. 4. Department of Social Medicine, Qazvin Univ. of Medical Sciences, Qazvin, Iran
  5. 5. UNICEF, UNDP/World Bank, WHO Spec. Prog. Res. Train. Trop. D., CH 1211, Geneva 27, Switzerland
  6. 6. Ferney-Voltaire 01210, 20 Avenue Vessy, France

Source: Vaccine Published:2005


Abstract

To establish the safety and reproducibility of live challenge with Leishmania major, a single frozen stabilate was used in two open, single-arm leishmanization (LZ) trials. A total of 42 inoculations in 28 male adult volunteers were followed until complete healing. Lesions induced by LZ are as diverse as natural infection, but much milder. Total protection was seen in 100% (11/11) of recovered volunteers. LZ used as live challenge to test vaccine candidates reduces the required sample size, is cost effective for surrogate markers studies and will induce protection in the participants who are not protected by candidate vaccines. © 2005 Elsevier Ltd. All rights reserved.
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