Efficient Media for High Lipase Production: One Variable at a Time Approach

Efficient Media for High Lipase Production: One Variable at a Time Approach

Soleymani S¹ ; Alizadeh H¹ ; Mohammadian H¹ ; Rabbani E³ ; Moazen F¹ ; Sadeghi HM¹ ; Shariat ZS² ; Etemadifar Z³ ; Rabbani M¹

Show Affiliations

1. Department of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2. Department of Biochemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
3. Department of Biology, Faculty of Basic Sciences, University of Isfahan, Isfahan, Iran

Source: Avicenna Journal of Medical Biotechnology Published:2017

Abstract

Background: Lipase enzymes have applications in a wide range of industries. A crucial determining factor of industrial prices of these enzymes is the culture media composition that is constantly under review by researchers. In this work, for maximum lipase production by Bacillus sp. ZR-5, culture media compositions were optimized using one variable at a time strategy. Methods: For this purpose, the culture medium parameters such as low and high cost carbon and nitrogen sources, substrates and incubation times were evaluated. Results: Maximum lipase activity was achieved after 24 hr of incubation with 1.5% of glucose syrup (1600±69.1 u/mg), 1% of fish powder (1238±36.7 u/mg) and olive oil (1407±2.1 u/mg) as low cost carbon and nitrogen sources and substrate, respectively. Conclusion: Our results show a significant increase in lipase activity with usage of low cost sources; this could help in reducing the media prices for industrial application of lipase enzyme. © 2017, Avicenna Journal of Medical Biotechnology. All rights reserved.

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Hamid Mirmohammad Sadeghi (5)

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Style	Citing Format
MLA	Soleymani S, et al.. "Efficient Media for High Lipase Production: One Variable at a Time Approach." Avicenna Journal of Medical Biotechnology, vol. 9, no. 2, 2017, pp. 82-86.
APA	Soleymani S, Alizadeh H, Mohammadian H, Rabbani E, Moazen F, Sadeghi HM, Shariat ZS, Etemadifar Z, Rabbani M (2017). Efficient Media for High Lipase Production: One Variable at a Time Approach. Avicenna Journal of Medical Biotechnology, 9(2), 82-86.
Chicago	Soleymani S, Alizadeh H, Mohammadian H, Rabbani E, Moazen F, Sadeghi HM, Shariat ZS, Etemadifar Z, Rabbani M. "Efficient Media for High Lipase Production: One Variable at a Time Approach." Avicenna Journal of Medical Biotechnology 9, no. 2 (2017): 82-86.
Harvard	Soleymani S et al. (2017) 'Efficient Media for High Lipase Production: One Variable at a Time Approach', Avicenna Journal of Medical Biotechnology, 9(2), pp. 82-86.
Vancouver	Soleymani S, Alizadeh H, Mohammadian H, Rabbani E, Moazen F, Sadeghi HM, et al.. Efficient Media for High Lipase Production: One Variable at a Time Approach. Avicenna Journal of Medical Biotechnology. 2017;9(2):82-86.
BibTex	@article{ author = {Soleymani S and Alizadeh H and Mohammadian H and Rabbani E and Moazen F and Sadeghi HM and Shariat ZS and Etemadifar Z and Rabbani M}, title = {Efficient Media for High Lipase Production: One Variable at a Time Approach}, journal = {Avicenna Journal of Medical Biotechnology}, volume = {9}, number = {2}, pages = {82-86}, year = {2017} }
RIS	TY - JOUR AU - Soleymani S AU - Alizadeh H AU - Mohammadian H AU - Rabbani E AU - Moazen F AU - Sadeghi HM AU - Shariat ZS AU - Etemadifar Z AU - Rabbani M TI - Efficient Media for High Lipase Production: One Variable at a Time Approach JO - Avicenna Journal of Medical Biotechnology VL - 9 IS - 2 SP - 82 EP - 86 PY - 2017 ER -

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