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Evaluating the Frequency of Resistance to Pyrazinamide Among Drug-Resistant Strains of Mycobacterium Tuberculosis in Isfahan, Iran Publisher



Nasresfahani B1 ; Moghim S1 ; Salehi M2 ; Keikha M3
Authors
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Authors Affiliations
  1. 1. Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Regional Tuberculosis Reference Laboratories, Isfahan, Iran
  3. 3. Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Source: Archives of Clinical Infectious Diseases Published:2021


Abstract

Background: Pyrazinamide is one of the most important first-line medications for the treatment of tuberculosis and an alternative intake for MDR-TB and XDR-TB patients. Objectives: The purpose of this study was to evaluate resistance to pyrazinamide in the isolates resistant to the Mycobacterium tuberculosis drug in patients in the city of Isfahan. Methods: In this study, the drug susceptibility test was performed with pyrazinamide using the proportion method and PZA assay on 47 isolates resistant to Mycobacterium tuberculosis. Then, the mutations of the pncA and rpsA genes of the isolates resistant to pyrazinamide were evaluated by the sequencing method. Results: According to the proportion method, 19 cases were resistant to pyrazinamide, 16 of which had mutations in their pncA and rpsA genes. Besides, five new mutations were recorded, and three isolates lacked mutations in the mentioned genes. Conclusions: Pyrazinamide resistance is high in MDR-TB and INH mono-resistant isolates. Therefore, evaluating the susceptibility to pyrazinamide in patients with MDR-TB before the initiation of treatment with pyrazinamide is considered essential. © 2021, Author(s).
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