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Antibacterial Effect of Nano-Chlorhexidine on Enterococcus Faecalis Biofilm in Root Canal System:An in Vitro Study Publisher



Hajihassani N1 ; Alavi O2 ; Karamshahi M3 ; Marashi SMA4 ; Khademi A6 ; Mohammadi N5
Authors
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Authors Affiliations
  1. 1. Department of Endodontics, Dental Caries Prevention Research Center, Qazvin University of Medical Sciences, Iran
  2. 2. Department of Endodontics, Qazvin University of Medical Sciences, Iran
  3. 3. Department of Periodontics, School of Dentistry, Qazvin University of Medical Sciences, Iran
  4. 4. Department of Microbiology, School of Medicine, Medical Microbiology Research Center, Qazvin University of Medical Sciences, Iran
  5. 5. Department of Community Medicine, School of Medicine, Children Growth Research Center, Research Institute for Non-Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran
  6. 6. Department of Endodontics, School of Dentistry, Dental Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Dental Research Journal Published:2022


Abstract

Background: A new method to improve the properties of the materials is nano-encapsulation, which improves the biological properties, antibacterial activity along with reduction of toxicity. Due to the spread of nano-knowledge, the present study was performed to evaluate the antibacterial effect of nano-chlorhexidine (CHX) on Enterococcus faecalis biofilm in the root canal system. Materials and Methods: In this in vitro experimental study, 55 matured single-root mandibular premolars were decoronated and the canals were prepared by single length method up to #F3 ProTaper Universal system. Five teeth were selected as negative control. Then, the teeth were randomly divided into three experimental groups (n = 15) and a positive control group (n = 5).The experimental groups were irrigated with 2% nano- CHX gel, 2% CHX solution, and 5.25% sodium hypochlorite (NaOCl), respectively. Finally, the number of colonies was counted. Kruskal-Wallis test was used to compare the number of colonies among groups.The level of significance was set at P < 0.05. Results: The mean number of colonies in the groups of nano-CHX, NaOCl, CHX, and positive control were obtained as 17.73 ± 18.69, 35.53 ± 36.42, 38.8 ± 31.8, and 96.8 ± 22.52, respectively. There was a significant decrease in the number of colonies in all the experimental groups compared to the control group (P < 0.05). However, difference in the number of colonies among these three groups was not significant (P > 0.05). Conclusion: The use of nano-CHX in removing E. faecalis biofilm from root canal is as effective as the use of CHX and NaOCl. © 2022 Dental Research Journal.
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