Allele Specific-Pcr and Melting Curve Analysis Showed Relatively High Frequency of Β-Casein Gene A1 Allele in Iranian Holstein, Simmental and Native Cows

Allele Specific-Pcr and Melting Curve Analysis Showed Relatively High Frequency of Β-Casein Gene A1 Allele in Iranian Holstein, Simmental and Native Cows Publisher Pubmed

Gholami M¹ ; Hafezian SH¹ ; Rahimi G¹ ; Farhadi A¹ ; Rahimi Z² ; Kahrizi D^{2, 3} ; Kiani S⁴ ; Karim H⁵ ; Vaziri S⁶ ; Muhammadi S⁵ ; Veisi F⁷ ; Ghadiri K⁸ ; Shetabi H⁹ ; Zargooshi J¹⁰

Show Affiliations

1. Laboratory for Molecular Genetics and Cytogenetics, Department of Animal Sciences, Faculty of Animal Sciences and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran
2. Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
3. Department of Agronomy and Plant Breeding, Razi University, Kermanshah, Iran
4. Research Institute of Applied Physics and Astronomy, University of Tabriz, Tabriz, Iran
5. Department of Cardiology, Kermanshah University of Medical Sciences, Kermanshah, Iran
6. Department of Infectious Diseases, Kermanshah University of Medical Sciences, Kermanshah, Iran
7. Department of Obstetrics and gynecology, Kermanshah University of Medical Sciences, Kermanshah, Iran
8. Nosocomial Infections Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran
9. Department of Anesthesiology, Isfahan University of Medical Sciences, Isfahan, Iran
10. Department of Sexual Medicine, The Rhazes Center for Research in Family Health and Sexual Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Source: Cellular and Molecular Biology Published:2016

Abstract

There are two allelic forms of A1 and A2 of β-casein gene in dairy cattle. Proteolytic digestion of bovine β-casein A1 type produces bioactive peptide of β-casomorphin-7 known as milk devil. β-casomorphin-7 causes many diseases, including type 1 diabetes, cardiovascular disease syndrome, sudden death and madness. The aim of the present study was to determine the different allelic forms of β-casein gene in Iranian Holstein, Simmental and native cattle in order to identify A1 and A2 variants. The blood samples were collected randomly and DNA was extracted using modified salting out method. An 854 bp fragment including part of exon 7 and part of intron 6 of β-casein gene was amplified by allele specific polymerase chain reaction (AS-PCR). Also, the accuracy of AS-PCR genotyping has been confirmed by melting temperature curve analysis using Real-time PCR machinery. The comparison of observed allele and genotype frequency among the studied breeds was performed using the Fisher exact and Chi-squared test, respectively by SAS program. Obtained results showed the A1 allele frequencies of 50, 51.57, 54.5, 49.4 and 46.6% in Holstein, Simmental, Sistani, Taleshi and Mazandarani cattle populations, respectively. The chi-square test was shown that no any populations were in Hardy-Weinberg equilibrium for studied marker locus. Comparison and analysis of the test results for allelic frequency showed no any significant differences between breeds (P > 0.05). The frequency of observed genotypes only differs significantly between Holstein and Taleshi breeds but no any statistically significant differences were found for other breeds (P > 0.05). A relatively high frequency of β-casein A1 allele was observed in Iranian native cattle. Therefore, determine the genotypes and preference alleles A2 in these native and commercial cattle is recommended.

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Style	Citing Format
MLA	Gholami M, et al.. "Allele Specific-Pcr and Melting Curve Analysis Showed Relatively High Frequency of Β-Casein Gene A1 Allele in Iranian Holstein, Simmental and Native Cows." Cellular and Molecular Biology, vol. 62, no. 12, 2016, pp. 138-143.
APA	Gholami M, Hafezian SH, Rahimi G, Farhadi A, Rahimi Z, Kahrizi D, Kiani S, Karim H, Vaziri S, Muhammadi S, Veisi F, Ghadiri K, Shetabi H, Zargooshi J (2016). Allele Specific-Pcr and Melting Curve Analysis Showed Relatively High Frequency of Β-Casein Gene A1 Allele in Iranian Holstein, Simmental and Native Cows. Cellular and Molecular Biology, 62(12), 138-143.
Chicago	Gholami M, Hafezian SH, Rahimi G, Farhadi A, Rahimi Z, Kahrizi D, Kiani S, et al.. "Allele Specific-Pcr and Melting Curve Analysis Showed Relatively High Frequency of Β-Casein Gene A1 Allele in Iranian Holstein, Simmental and Native Cows." Cellular and Molecular Biology 62, no. 12 (2016): 138-143.
Harvard	Gholami M et al. (2016) 'Allele Specific-Pcr and Melting Curve Analysis Showed Relatively High Frequency of Β-Casein Gene A1 Allele in Iranian Holstein, Simmental and Native Cows', Cellular and Molecular Biology, 62(12), pp. 138-143.
Vancouver	Gholami M, Hafezian SH, Rahimi G, Farhadi A, Rahimi Z, Kahrizi D, et al.. Allele Specific-Pcr and Melting Curve Analysis Showed Relatively High Frequency of Β-Casein Gene A1 Allele in Iranian Holstein, Simmental and Native Cows. Cellular and Molecular Biology. 2016;62(12):138-143.
BibTex	@article{ author = {Gholami M and Hafezian SH and Rahimi G and Farhadi A and Rahimi Z and Kahrizi D and Kiani S and Karim H and Vaziri S and Muhammadi S and Veisi F and Ghadiri K and Shetabi H and Zargooshi J}, title = {Allele Specific-Pcr and Melting Curve Analysis Showed Relatively High Frequency of Β-Casein Gene A1 Allele in Iranian Holstein, Simmental and Native Cows}, journal = {Cellular and Molecular Biology}, volume = {62}, number = {12}, pages = {138-143}, year = {2016} }
RIS	TY - JOUR AU - Gholami M AU - Hafezian SH AU - Rahimi G AU - Farhadi A AU - Rahimi Z AU - Kahrizi D AU - Kiani S AU - Karim H AU - Vaziri S AU - Muhammadi S AU - Veisi F AU - Ghadiri K AU - Shetabi H AU - Zargooshi J TI - Allele Specific-Pcr and Melting Curve Analysis Showed Relatively High Frequency of Β-Casein Gene A1 Allele in Iranian Holstein, Simmental and Native Cows JO - Cellular and Molecular Biology VL - 62 IS - 12 SP - 138 EP - 143 PY - 2016 ER -

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