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Lithium Chloride Can Induce Differentiation of Human Immortalized Renvm Cells Into Dopaminergic Neurons



Soleimani M1 ; Ghasemi N1
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Authors Affiliations
  1. 1. Department of Anatomical Sciences and Molecular Biology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Source: Avicenna Journal of Medical Biotechnology Published:2017

Abstract

Background: Stem cell-based therapy is a novel strategy for the treatment of neuro-degenerative diseases. The transplantation of fully differentiated cells instead of stem cells in order to decrease serious adverse complications of stem cell therapy is a new idea. In this study, the effect of lithium chloride on dopaminergic differentiation of human immortalized RenVm cells was investigated in order to access a population of fully differentiated cells for transplantation in Parkinson disease. Methods: The immortalized RenVm cells were induced to dopaminergic differentiation using a neurobasal medium supplemented with N2 and different concentrations (1, 3, 6 mM) of Lithium Chloride (LiCl) for 4, 8 and 12 days. The efficiency of dopaminergic differentiation was evaluated using immunocytochemistry and western blot techniques for tyrosine hydroxylase and β-catenin marker expression. Results: Our results indicated that LiCl can promote dopaminergic differentiation of RenVm cells in a dose-dependent manner. Conclusion: It can be concluded that LiCl is able to facilitate dopaminergic differentiation of cultured cells by affecting Wnt-frizzled signaling pathway. © 2017, Avicenna Journal of Medical Biotechnology. All rights reserved.
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Nazem Ghasemi (6)
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