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Production of Volatile Sulphides in Allium Porrum Cell Cultures



Asghari G1, 3 ; Lockwood GB2 ; Houshfar G1
Authors
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Authors Affiliations
  1. 1. Faculty of Pharmaceutical Sciences, Isfahan Univ. of Medical Sciences, Isfahan, Iran
  2. 2. Sch. of Pharm./Pharmaceutical Sci., University of Manchester, Manchester, United Kingdom
  3. 3. Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Isfahan Univ. of Medical Sciences, Isfahan, PO Box 81745-359, Iran

Source: Daru Published:2002

Abstract

Production of volatile sulphides in cell cultures of Allium porrum is described. Allium porrum calluses were initiated from whole seedlings. The high growth rate of Allium porrum callus was achived in Murashige and Skoog media containing only 1ppm 2,4-Dichlorophenoxy acetic acid. The routine method of solvent extraction of volatile sulphides was used for Allium porrum and the concentrated extract was subjected to capillary GC and GC-MS. Dipropyl disulphide and 4-methyl thiazolethanol were identified in A. porrum aggregated suspension cells.