Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Blockage of Mir-92A-3P With Locked Nucleic Acid Induces Apoptosis and Prevents Cell Proliferation in Human Acute Megakaryoblastic Leukemia Publisher Pubmed



Sharifi M1 ; Salehi R1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, 81744-176, Iran

Source: Cancer Gene Therapy Published:2016


Abstract

MicroRNAs (miRNAs) are non-coding RNAs involved in post-transcriptional regulation of gene expression. In many cancers, up- or downregulation of different miRNAs is reported. In acute myeloid leukemia, upregulation of miR-92a-3p was reported in human in vitro studies. We performed blockage of miR-29a-3p in human acute megakaryoblastic leukemia cell line (M-07e) by using locked nucleic acid (LNA) and cell proliferation; apoptosis and necrosis were assessed. At different time points after LNA-anti-miR92a-3p transfection, miR-92a-3p quantitation was assessed by qRT-real-time PCR, MTT assay and annexin/propidium iodide staining were performed. The data were processed using the ANOVA test. At all three time points, the expression of miR-92a-3p was lower in the LNA-anti-miR group compared with the control groups. Cell viability between LNA-Anti-miR and the control group was statistically significant. Blockage of miR-92a-3p was associated with increment of the ratio of apoptotic cells in the LNA-anti-miR group was higher than the other group. The ratio of necrotic cells in the LNA-antimiR group was higher than the other groups. These assessments indicate that miR-92a-3p blockage can decrease the viability of M-07e cells, which is mainly due to induction of apoptosis and necrosis. Our findings could open up a path to a miRNA based therapeutic approach for treatment of acute megakaryoblastic leukemia. © 2016 Nature America, Inc. All rights reserved 0929-1903/16.
Related Docs
View other Related Docs
1. Inhibition of Microrna Mir-92A Inhibits Cell Proliferation in Human Acute Promyelocytic Leukemia; [Mikrorna Mir-92A Inhibisyonu Insan Akut Promyelositik Losemide Hucre Proliferasyonunu Inhibe Eder], Turkish Journal of Hematology (2013)
2. Inhibition of Microrna Mir-92A Induces Apoptosis and Inhibits Cell Proliferation in Human Acute Promyelocytic Leukemia Through Modulation of P63 Expression, Molecular Biology Reports (2014)
3. Apoptosis-Inducing and Antiproliferative Effect by Inhibition of Mir-182-5P Through the Regulation of Casp9 Expression in Human Breast Cancer, Cancer Gene Therapy (2017)
4. Apoptosis Induction in Acute Promyelocytic Leukemia Cells Through Upregulation of Cebpα by Mir-182 Blockage, Molecular Biology Research Communications (2018)
5. Inducing Apoptosis and Decreasing Cell Proliferation in Human Acute Promyelocytic Leukemia Through Regulation Expression of Casp3 by Let-7A-5P Blockage, Indian Journal of Hematology and Blood Transfusion (2018)
6. Inducing Cell Proliferative Prevention in Human Acute Promyelocytic Leukemia by Mir-182 Inhibition Through Modulation of Casp9 Expression, Biomedicine and Pharmacotherapy (2017)
7. Inhibition of Microrna Mir-222 With Lna Inhibitor Can Reduce Cell Proliferation in B Chronic Lymphoblastic Leukemia, Indian Journal of Hematology and Blood Transfusion (2017)
8. Degradation of Mir-21 Induces Apoptosis and Inhibits Cell Proliferation in Human Hepatocellular Carcinoma, Cancer Gene Therapy (2015)
Experts (# of related papers)
View all Related Experts
Mohammadreza Sharifi (35)
Rasoul Salehi (13)
Other Related Docs
9. Locked Nucleic Acid Anti-Mir-21 Inhibits Cell Growth and Invasive Behaviors of a Colorectal Adenocarcinoma Cell Line: Lna-Anti-Mir As a Novel Approach, Cancer Gene Therapy (2016)
10. Induction of Apoptosis and Necrosis in Human Acute Erythroleukemia Cells by Inhibition of 47Tlong Non-Coding Rna Pvt1, Molecular Biology Research Communications (2018)
11. Inducing Apoptosis and Decreases Cell Proliferation in Human Breast Cancer Cells Through Mir-182-5P Blockage Caused by Locked Nucleic Acid, Journal of Isfahan Medical School (2017)
12. Differential Expression Profile of Mir-27B, Mir-29A, and Mir-155 in Chronic Lymphocytic Leukemia and Breast Cancer Patients, Molecular Therapy Oncolytics (2020)
13. Microrna in Leukemia: Tumor Suppressors and Oncogenes With Prognostic Potential, Journal of Cellular Physiology (2019)
14. Antiproliferative Effect of Upregulation of Hsa-Let-7C-5P in Human Acute Erythroleukemia Cells, Cytotechnology (2018)
15. Reduce Proliferation of Human Bone Marrow Cells From Acute Myeloblastic Leukemia With Minimally Differentiation by Blocking Lncrna Pvt1, Clinical and Translational Oncology (2020)
16. Lna Inhibitor in Microrna Mir-23B As a Potential Anti-Proliferative Option in Human Hepatocellular Carcinoma, Journal of Gastrointestinal Cancer (2020)
17. The Mir-125A-3P Inhibits Tim-3 Expression in Aml Cell Line Hl-60 in Vitro, Indian Journal of Hematology and Blood Transfusion (2017)
18. Locked Nucleic Acid Inhibits Mir-92A-3P in Human Colorectal Cancer, Induces Apoptosis and Inhibits Cell Proliferation, Cancer Gene Therapy (2016)
19. Evaluation of the Effect of Tim-3 Suppression by Mir-498 and Its Effect on Apoptosis and Proliferation Rate of Hl-60 Cell Line, Pathology Research and Practice (2018)
20. Inhibition of Microrna-21 Via Locked Nucleic Acid-Anti-Mir Suppressed Metastatic Features of Colorectal Cancer Cells Through Modulation of Programmed Cell Death 4, Tumor Biology (2017)
21. Downregulation of Mir100hg Induces Apoptosis in Human Megakaryoblastic Leukemia Cells, Indian Journal of Hematology and Blood Transfusion (2021)
22. Current Status and Perspectives Regarding Lna-Anti-Mir Oligonucleotides and Microrna Mir-21 Inhibitors As a Potential Therapeutic Option in Treatment of Colorectal Cancer, Journal of Cellular Biochemistry (2017)
23. Effects of Hsa-Pir-32877 Suppression With Antisense Lna Gapmers on the Proliferation and Apoptosis of Human Acute Myeloid Leukemia Cells, International Journal of Molecular and Cellular Medicine (2023)
24. Negative Regulation of Tim-3 Expression in Aml Cell Line (Hl-60) Using Mir-330-5P, British Journal of Biomedical Science (2016)
25. Circulating Microrna-192 As a Diagnostic Biomarker in Human Chronic Lymphocytic Leukemia, Cancer Gene Therapy (2016)
26. Mir-939, As an Important Regulator in Various Cancers Pathogenesis, Has Diagnostic, Prognostic, and Therapeutic Values: A Review, Journal of the Egyptian National Cancer Institute (2024)
27. Mir-574, Mir-499, Mir-125B, Mir-106A, and Mir-9 Potentially Target Tgfbr-1 and Tgfbr-2 Genes Involving in Inflammatory Response Pathway: Potential Novel Biomarkers for Chronic Lymphocytic Leukemia, Pathology Research and Practice (2022)
28. Evaluation of Silencing Effect of Mir-133A-5P Mimic on Tim-3 Expression in Aml (Hl-60) Cell Line, Indian Journal of Clinical Biochemistry (2020)
29. Circulating Lncrna Hotair Is a Biomarker for Pediatric Acute Lymphoblastic Leukemia and Mediator of Mir-326 Exosomal Export, Scientific Reports (2025)
30. The Prominent Role of Mir-942 in Carcinogenesis of Tumors, Advanced Biomedical Research (2022)
31. Evaluation of Microrna Expression Pattern (Mir-28, Mir-181A, Mir-34A, and Mir-31) in Patients With Covid-19 Admitted to Icu and Diabetic Covid-19 Patients, Intervirology (2023)
32. Evaluation of the Expressed Mir‑129 and Mir‑549A in Patients With Multiple Sclerosis, Advanced Biomedical Research (2021)
33. Pbmcs: A New Source of Diagnostic and Prognostic Biomarkers, Archives of Physiology and Biochemistry (2022)
34. The Effect of Decitabine on the Expression and Methylation of the Ppp1ca, Btg2, and Pten in Association With Changes in Mir-125B, Mir-17, and Mir-181B in Nalm6 Cell Line, Journal of Cellular Biochemistry (2019)
35. Recent Insights Into the Microrna-Dependent Modulation of Gliomas From Pathogenesis to Diagnosis and Treatment, Cellular and Molecular Biology Letters (2022)
36. Transcription Analysis of a Histones Modifiers Panel Coupled With Critical Tumor Suppressor Genes Displayed Frequent Changes in Patients With Aml.: Mrna Levels of Histones Modifiers and Tsgs in Aml, Current Research in Translational Medicine (2021)
37. Modulation of Long Non-Coding Rnas and Micrornas by Quercetin As a Potential Therapeutical Approach in Cancer: A Comprehensive Review, Current medicinal chemistry (2025)
38. The Importance of Snps at Mirna Binding Sites As Biomarkers of Gastric and Colorectal Cancers: A Systematic Review, Journal of Personalized Medicine (2022)
39. Upregulation of Mir-222 in Both Helicobacter Pylori-Infected and Noninfected Gastric Cancer Patients, Journal of Genetics (2016)
40. Knockdown of Sirlnt by Antisense Lna Gapmers and Evaluation of Its Inhibitory Effect on Proliferation and Apoptosis in Mcf7 Cell Line, Indian Journal of Gynecologic Oncology (2023)
41. Retraction Note To: Inhibition of Microrna Mir-92A Induces Apoptosis and Inhibits Cell Proliferation in Human Acute Promyelocytic Leukemia Through Modulation of P63 Expression (Molecular Biology Reports, (2014), 41, 5, (2799-2808), 10.1007/S11033-014-3134-5), Molecular Biology Reports (2019)
42. Unveiling Mesenchymal Stem Cells’ Regenerative Potential in Clinical Applications: Insights in Mirna and Lncrna Implications, Cells (2023)
43. Exploring Plasma Expression Levels of Mir-200C-3P, Mir-150, and Mir-155 in Sars-Cov-2 Positive and Negative Subjects, Future Virology (2024)
44. Microrna Profiles in Critically Ill Patients, Current Medicinal Chemistry (2024)
45. Blockage of Hotair Reduced Cell Proliferation in Human Ovarian Cancer Cells Through Upregulation of Akt2, Indian Journal of Gynecologic Oncology (2019)
46. Long Non-Coding Rnas As Potential Biomarkers or Therapeutic Targets in Gastric Cancer, Gastroenterology and Hepatology from Bed to Bench (2023)
47. Lncrna Flvcr1-As1 Sponges Mir-381-3P and Promotes Wnt Signaling Pathway Resulting in Colorectal Cancer Progression, Egyptian Journal of Medical Human Genetics (2025)
48. Comparison of Mir-211 Expression in Murine Melanoma Cell Line and Murine Melanoma Tumor by Real-Time Polymerase Chain Reaction, Journal of Isfahan Medical School (2012)
49. The Significant Role of Micrornas in Gliomas Angiogenesis: A Particular Focus on Molecular Mechanisms and Opportunities for Clinical Application, Cellular and Molecular Neurobiology (2023)
50. Deciphering the Multifaceted Role of Micrornas in Hepatocellular Carcinoma: Integrating Literature Review and Bioinformatics Analysis for Therapeutic Insights, Heliyon (2024)