Isfahan University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Anti-Proliferative and Anti-Apoptotic Effects of Grape Seed Extract on Chemo-Resistant Ovcar-3 Ovarian Cancer Cells Publisher



Homayoun M1 ; Ghasemnezhad Targhi R2 ; Soleimani M1
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Radiology, Imam Reza Hospital, Mashhad University of Medical Sciences, Mashhad, Iran

Source: Research in Pharmaceutical Sciences Published:2020


Abstract

Background and purpose: Ovarian cancer is the deadliest cancer in women. The main challenge in the inhibition of ovarian cancer cells is chemo-resistance. Seeking to overcome this issue, several strategies have been suggested, including the administration of natural products. Grape seed extract (GSE) is a good source of polyphenols and its anticancer effects have been reported by many studies. In this study we aimed to evaluate the effects of GSE on OVCAR-3, a chemo-resistant ovarian cancer line. Experimental approach: OVCAR-3 cells were treated with GSE (71 μg/mL) for 24 and 48 h. Cell viability and cell apoptosis were measured by MTT and flow cytometry. The real-time polymerase chain reaction was used to determine the expression of genes involved in the cell cycle (PTEN, DACT1, AKT, MTOR, GSK3B, C-MYC, CCND1, and CDK4) and apoptosis (BAX, BCl2, CASP3, 8 and 9). The expression of CASP3 protein was evaluated by the CASP3 assay. Findings / Results: The results showed that treatment of OVCAR-3 cells with GSE, increased the expression level of PTEN and DACT1 tumor suppressor genes, as well as apoptotic genes, CASP3, 8, and 9 (P < 0.001). Also, the induction of tumor suppressor genes expression was associated with an increase in the expression of BAX/BCL2 gene ratio as pro- and anti-apoptotic genes. The expression of the genes involved in the cell cycle, CCND1 and CDK4, was inhibited (P < 0.001). The results indicated that GSE induced cell apoptosis in a time-dependent manner (P < 0.001). Also, the GSE treatment resulted in the CASP3 protein expression (P < 0.001). Conclusion and implications: According to the results of this study, GSE may exert anti-tumorigenic effects on chemo-resistant OVCAR-3 ovarian cancer cells which might be mediated by the expression of tumor suppressor genes that interact with cell signaling pathways, cell cycle, and cell apoptosis. Hence, the consumption of GSE extract during chemotherapy may overcome part of chemo-resistance in ovarian cancer. © 2020 Wolters Kluwer Medknow Publications. All rights reserved.
Related Docs
View other Related Docs
1. In Vitro Evaluation of the Pogostone Effects on the Expression of Pten and Dact1 Tumor Suppressor Genes, Cell Cycle, and Apoptosis in Ovarian Cancer Cell Line, Research in Pharmaceutical Sciences (2022)
2. Expression Assay of Pten, Cdk4, and Mtor Genes and Cell Proliferation in Breast Cancer Cell Line (T47d) Under Grape Seed Extract Treatment, Journal of Isfahan Medical School (2022)
3. Estrogen Receptor Beta (Erβ) May Act As Mediator in Apoptotic Induction of Grape Seed Extract (Gse), Asian Pacific Journal of Cancer Prevention (2019)
4. Mirnas: Emerging Agents for Therapeutic Effects of Polyphenols on Ovarian Cancer, Mini-Reviews in Medicinal Chemistry (2024)
5. All-Trans Retinoic Acid and Genistein Induce Cell Apoptosis in Ovcar-3 Cells by Increasing the P14 Tumor Suppressor Gene, Research in Pharmaceutical Sciences (2016)
6. The Emerging Role of Circular Rnas in Cisplatin Resistance in Ovarian Cancer: From Molecular Mechanism to Future Potential, Non-coding RNA Research (2024)
7. The Potential Anti-Cancer Effects of Quercetin on Blood, Prostate and Lung Cancers: An Update, Frontiers in Immunology (2023)
8. New Diterpene Compound From Euphorbia Connate Boiss., 3,7,14,15-Tetraacetyl-5-Propanoyl-13(17)-Epoxy-8,10(18)-Myrsinadiene, Inhibits the Growth of Ovarian Cancer Cells by Promoting Mitochondrial-Mediated Apoptosis, Nutrition and Cancer (2021)
Experts (# of related papers)
View all Related Experts
Mahmoud Aghaei (10)
Mustafa Ghanadian (6)
Other Related Docs
9. 15-Hydroxy-8(17),13(E)-Labdadiene-19-Carboxylic Acid (Hlca) Inhibits Proliferation and Induces Cell Cycle Arrest and Apoptosis in Ovarian Cancer Cells, Life Sciences (2021)
10. Regulating Mirnas Expression by Resveratrol: Novel Insights Based on Molecular Mechanism and Strategies for Cancer Therapy, Current Molecular Pharmacology (2024)
11. Persianolide-A, an Eudesmanolide-Type Sesquiterpene Lactone From Artemisia Kopetdaghensis, Induces Apoptosis by Regulating Erk Signaling Pathways, Research in Pharmaceutical Sciences (2024)
12. Role of Peroxisome Proliferator-Activated Receptor Alpha and Gamma in Antiangiogenic Effect of Pomegranate Peel Extract, Iranian Journal of Basic Medical Sciences (2016)
13. Cytotoxic Activities of Euphorbia Kopetdaghi Against Ovcar-3 and Ej-138 Cell Lines, Journal of HerbMed Pharmacology (2015)
14. Natural and Herbal Compounds Targeting Breast Cancer, a Review Based on Cancer Stem Cells, Iranian Journal of Basic Medical Sciences (2020)
15. Α2β1 Integrin Specific Inhibitor Btt-3033 Promotes Paclitaxel-Induced Apoptosis in Human Ovarian Cancer Cells, Research in Pharmaceutical Sciences (2024)
16. Effect of Epigallocatechin-3-Gallate (Egcg) on Cell Proliferation Inhibition and Apoptosis Induction in Lymphoblastic Leukemia Cell Line, Journal of HerbMed Pharmacology (2015)
17. Mechanisms of Apoptosis-Related Non-Coding Rnas in Ovarian Cancer: A Narrative Review, Apoptosis (2025)
18. Cytotoxicity Effects of Ethanolic Extract of Punica Granatum Var. Pleniflora on Mcf-7 Compared With L929 Cells, New Armenian Medical Journal (2023)
19. Ovarian Cancer Risk and Nonisoflavone Flavonoids Intake: A Systematic Review of Epidemiological Studies, Journal of Research in Medical Sciences (2016)
20. Evidences for Involvement of Estrogen Receptor Induced Erk1/2 Activation in Ovarian Cancer Cell Proliferation by Cadmium Chloride, Toxicology in Vitro (2019)
21. Myricetin Apoptotic Effects on T47d Breast Cancer Cells Is a P53-Independent Approach, Asian Pacific Journal of Cancer Prevention (2020)
22. Cytotoxicity Evaluation of Synthesized Silver Nanoparticles by a Green Method Against Ovarian Cancer Cell Lines, Nanomedicine Research Journal (2022)
23. Kopetdaghinanes, Pro-Apoptotic Hemiacetialic Cyclomyrsinanes From Euphorbia Kopetdaghi, Fitoterapia (2020)
24. Blockage of Hotair Reduced Cell Proliferation in Human Ovarian Cancer Cells Through Upregulation of Akt2, Indian Journal of Gynecologic Oncology (2019)
25. Resveratrol Enhances Sensitivity of Renal Cell Carcinoma to Tivozanib: An In-Vitro Study, Tissue and Cell (2024)
26. Inhibitory Effect of Genistein on Plc/Prf5 Hepatocellular Carcinoma Cell Line, International Journal of Preventive Medicine (2015)
27. Quercetin and Ovarian Cancer: An Evaluation Based on a Systematic Review, Journal of Research in Medical Sciences (2016)
28. Preventive Effects of Achillea Millefolium, Rosa Damascena and Origanum Majorana Hydroalcoholic Extracts on Breast Cancer in Female Mice, Current Cancer Therapy Reviews (2023)
29. The Modulatory Effects of Two Bioflavonoids, Quercetin and Thymoquinone on the Expression Levels of Dna Damage and Repair Genes in Human Breast, Lung and Prostate Cancer Cell Lines, Pathology Research and Practice (2022)
30. Jatropha-6(17),11 E -Diene Class Derivatives Induce Apoptosis Effects in Ovcar-3 and Caov-4 Ovarian Cancer Cell Lines Via a Mitochondrial Pathway, Biochemistry and Cell Biology (2017)
31. Adenosine Induces Cell Cycle Arrest and Apoptosis Via Cyclind1/Cdk4 and Bcl-2/Bax Pathways in Human Ovarian Cancer Cell Line Ovcar-3, Tumor Biology (2013)
32. Effect of Genistein on Apoptosis and Proliferation of Hepatocellular Carcinoma Hepa1‑6 Cell Line, International Journal of Preventive Medicine (2018)
33. Silibinin Exerts Anti-Cancer Activity on Human Ovarian Cancer Cells by Increasing Apoptosis and Inhibiting Epithelial-Mesenchymal Transition (Emt), Gene (2022)
34. The Therapeutic Effects and Mechanisms of Action of Resveratrol on Polycystic Ovary Syndrome: A Comprehensive Systematic Review of Clinical, Animal and in Vitro Studies, Clinical and Experimental Pharmacology and Physiology (2022)
35. Molecular Mechanisms of Drug Resistance in Ovarian Cancer, Journal of Cellular Physiology (2018)
36. Comparison of the Apoptosis Induction Effect of Pomegranate Peel Extract and Human Umbilical Vein Endothelial Cells on Melanoma, Journal of Isfahan Medical School (2016)
37. Galectin-9 Induces Apoptosis in Ovcar-3 Ovarian Cancer Cell Through Mitochondrial Pathway, Research in Pharmaceutical Sciences (2018)
38. Microrna-30C-2-3P Regulates Er Stress and Induces Apoptosis in Ovarian Cancer Cells Underlying Er Stress, EXCLI Journal (2021)
39. Therapeutic Potential of Quercetin on Human Breast Cancer in Different Dimensions, Inflammopharmacology (2020)
40. Glucosamine Reverses Drug Resistance in Mrp2 Overexpressing Ovarian Cancer Cells, European Journal of Pharmacology (2020)
41. The Effects of Chitosan-Loaded Jq1 Nanoparticles on Ovcar-3 Cell Cycle and Apoptosis-Related Gene Expression, Research in Pharmaceutical Sciences (2024)