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Mycobacterium Iranicum Sp. Nov., a Rapidly Growing Scotochromogenic Species Isolated From Clinical Specimens on Three Different Continents Publisher Pubmed



Shojaei H1 ; Daley C2 ; Gitti Z3 ; Hashemi A1 ; Heidarieh P1 ; Moore ERB4 ; Naser AD1 ; Russo C5 ; Van Ingen J6 ; Tortoli E7
Authors
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Authors Affiliations
  1. 1. Isfahan University of Medical Sciences, Isfahan, Iran
  2. 2. Department of Mycobacterial and Respiratory Infections, National Jewish Health, Denver, CO, United States
  3. 3. Department of Clinical Bacteriology, Parasitology, Zoonoses and Geographical Medicine, University Hospital of Heraklion, Heraklion, Greece
  4. 4. Department of Infectious Disease, Culture Collection University of Gothenburg (CCUG), Sahlgrenska Academy of the University of Gothenburg, Gothenburg, Sweden
  5. 5. Virology Laboratory, Bambino Gesu Children's Hospital, Rome, Italy
  6. 6. Department of Medical Microbiology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands
  7. 7. Emerging Bacterial Pathogens Unit, San Raffaele Scientific Institute, Milan, Italy

Source: International Journal of Systematic and Evolutionary Microbiology Published:2013


Abstract

The isolation and characterization of a novel, rapidly growing, scotochromogenic mycobacterial species is reported. Eight independent strains were isolated from clinical specimens from six different countries of the world, two in Iran, two in Italy and one in each of following countries: Greece, the Netherlands, Sweden and the USA. Interestingly, two of the strains were isolated from cerebrospinal fluid. The strains were characterized by rapid growth and presented orangepigmented scotochromogenic colonies. DNA-based analysis revealed unique sequences in the four regions investigated: the 16S rRNA gene, the rRNA gene internal transcribed spacer 1 and the genes encoding the 65 kDa heat-shock protein and the beta-subunit of RNA polymerase. The phylogenetic analysis placed the strains among the rapidly growing mycobacteria, being most closely related to Mycobacterium gilvum. The genotypic and phenotypic data both strongly supported the inclusion of the strains investigated here as members of a novel species within the genus Mycobacterium; the name Mycobacterium iranicum sp. nov. is proposed to indicate the isolation in Iran of the first recognized strains. The type strain is M05T (=DSM 45541T=CCUG 62053T=JCM 17461T). © 2013 IUMS Printed in Great Britain.
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