Mutant Soluble Interleukin-6 Receptor As a Potential Agent for Inhibiting Cell Migration and Inducing Apoptosis in a Breast Cancer Cell Line Publisher Pubmed



Mousavizadehmarvast MS ; Mirian M ; Shafiee F
Source: Molecular Biology Reports Published:2025

Abstract

Objectives: Interleukin-6 (IL-6) plays a crucial role in regulating of survival, invasion, and cell cycle of breast cancer cells. So, it appears that the suppression of IL-6 may function as a potential therapeutic agent in the context of cancer treatment. The aim of the present study is to investigate the effect of a mutated form of the soluble IL-6 receptor (mIL-6R) on the invasive ability and induction of apoptosis in MDA-MB-231 cancer cells. Methods: mIL-6R was expressed and purified in E. coli BL21 (DE3) bacteria and IMPACT system, respectively. The cytotoxic effects, inhibition of cell migration and apoptosis induction of mIL-6R were evaluated using MTT Assay, Scratch Assay, and Flow Cytometry assay, respectively, on the MDA-MB-231 cell line. Results: At a concentration of IC50 (7.41 µg.mL−1), mIL-6R inhibits cell migration and induces apoptosis, while at higher concentrations (2.IC50, 14.82 µg.mL−1), it leads to the activation of compensatory pathways and increased cell necrosis. Furthermore, disruption in the S phase of the cell cycle is observed, which may lead to DNA damage and cellular stress. mIL-6R exhibits multifaceted effects on cytotoxicity, cell migration, apoptosis, and cell cycle in a concentration-dependent manner. At IC50 (7.41 µg.mL−1), this protein primarily induced cells to enter the S phase and replicate their DNA, while at higher concentrations, such as 2.IC50 (14.82 µg.mL−1), cell cycle disruption and necrosis induction were observed. Conclusion: It appears that mIL-6R can act as a therapeutic candidate for breast cancer, but further investigations in animal models and clinical trials are required to determine the optimal dose and long-term effects. © 2025 Elsevier B.V., All rights reserved.