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Vegf and Mmp-9 Gene Expression Caused by Treatment With Helicobacter Pylori Neutrophil-Activating Recombinant Protein in a Breast Cancer Model



Soleimani N1 ; Farhangi B2 ; Mohabati Mobarez A1 ; Etyabi F3 ; Hassan Saraf Z4
Authors
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Authors Affiliations
  1. 1. Department of Bacteriology, Tarbiat Modares University, Tehran, Iran
  2. 2. Department of Genetics, Tarbiat Modares University, Tehran, Iran
  3. 3. Nanotechnology Research Centre, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Immunology, Tarbiat Modares University, Tehran, Iran

Source: Journal of Babol University of Medical Sciences Published:2015

Abstract

Background and objective: Breast cancer is a major cause of death in women, worldwide. Cytotoxic drugs may lead to various adverse side-effects in patients. Helicobacter pylori neutrophil-activating protein (HP-NAP) is one of the most important proteins, produced by helicobacter pylori. The purpose of this study was to investigate the expression of VEGF and MMP-9 genes, caused by HP-NAP treatment in a breast cancer model. Methods: In this experimental study, 18 female BALB/c mice, aged 6-8 weeks (19-23 g), were used. The mice were allocated to three groups: test group (treated with recombinant HP-NAP), positive control group (treated with an anti-cancer drug called trastuzumab), and negative control group (receiving phosphate-buffered saline). Expression and purification of HP-NAP were performed using nickel-resin affinity chromatography (Qiagen). The mice with breast tumors were treated with HP-NAP. MMP-9 and VEGF gene expression was measured using real-time polymerase chain reaction (PCR). Findings: Protein expression was confirmed, using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The analysis showed the expression of a 20, 000 dalton protein. Moreover, MMP-9 and VEGF gene expression decreased more significantly in the test group (due to treatment with HP-NAP), compared to the negative control group. The reduced expression in the test group was almost a quarter of the expression in the control group (p<0.01). Conclusion: The results showed that HP-NAP can reduce the expression of genes involved in metastasis in a mouse model of breast cancer. Therefore, this protein can be exploited for future therapeutic strategies in cancer treatment. © 2015, Babol University of Medical Sciences. All right reserved.
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