The Rapid and Sensitive Quantitative Determination of Galactose by Combined Enzymatic and Colorimetric Method: Application in Neonatal Screening

The Rapid and Sensitive Quantitative Determination of Galactose by Combined Enzymatic and Colorimetric Method: Application in Neonatal Screening Publisher Pubmed

Kianmehr A^{1, 2} ; Mahrooz A³ ; Ansari J⁴ ; Oladnabi M^{1, 5} ; Shahbazmohammadi H^{6, 7}

Show Affiliations

1. Biochemistry and Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran
2. Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran
3. Immunogenetic Research Center, Mazandaran University of Medical Sciences, Sari, Iran
4. Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
5. Department of Medical Genetics, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran
6. Department of Biology, Bandar Jask Branch, Islamic Azad University, Bandar Jask, Iran
7. Enzyme Technology Laboratory, Genetic and Metabolism Research Group, Pasteur Institute of Iran, Tehran, Iran

Source: Applied Biochemistry and Biotechnology Published:2016

Abstract

The quantitative measurement of galactose in blood is essential for the early diagnosis, treatment, and dietary monitoring of galactosemia patients. In this communication, we aimed to develop a rapid, sensitive, and cost-effective combined method for galactose determination in dry blood spots. This procedure was based on the combination of enzymatic reactions of galactose dehydrogenase (GalDH), dihydrolipoyl dehydrogenase (DLD), and alkaline phosphates with a colorimetric system. The incubation time and the concentration of enzymes used in new method were also optimized. The analytical performance was studied by the precision, recovery, linearity, and sensitivity parameters. Statistical analysis was applied to method comparison experiment. The regression equation and correlation coefficient (R2) were Y = 0.0085x + 0.032 and R2 = 0.998, respectively. This assay exhibited a recovery in the range of 91.7–114.3 % and had the limit detection of 0.5 mg/dl for galactose. The between-run coefficient of variation (CV) was between 2.6 and 11.1 %. The within-run CV was between 4.9 and 9.2 %. Our results indicated that the new and reference methods were in agreement because no significant biases exist between them. Briefly, a quick and reliable combined enzymatic and colorimetric assay was presented for application in newborn mass screening and monitoring of galactosemia patients. © 2016, Springer Science+Business Media New York.

Style	Citing Format
MLA	Kianmehr A, et al.. "The Rapid and Sensitive Quantitative Determination of Galactose by Combined Enzymatic and Colorimetric Method: Application in Neonatal Screening." Applied Biochemistry and Biotechnology, vol. 179, no. 2, 2016, pp. 283-293.
APA	Kianmehr A, Mahrooz A, Ansari J, Oladnabi M, Shahbazmohammadi H (2016). The Rapid and Sensitive Quantitative Determination of Galactose by Combined Enzymatic and Colorimetric Method: Application in Neonatal Screening. Applied Biochemistry and Biotechnology, 179(2), 283-293.
Chicago	Kianmehr A, Mahrooz A, Ansari J, Oladnabi M, Shahbazmohammadi H. "The Rapid and Sensitive Quantitative Determination of Galactose by Combined Enzymatic and Colorimetric Method: Application in Neonatal Screening." Applied Biochemistry and Biotechnology 179, no. 2 (2016): 283-293.
Harvard	Kianmehr A et al. (2016) 'The Rapid and Sensitive Quantitative Determination of Galactose by Combined Enzymatic and Colorimetric Method: Application in Neonatal Screening', Applied Biochemistry and Biotechnology, 179(2), pp. 283-293.
Vancouver	Kianmehr A, Mahrooz A, Ansari J, Oladnabi M, Shahbazmohammadi H. The Rapid and Sensitive Quantitative Determination of Galactose by Combined Enzymatic and Colorimetric Method: Application in Neonatal Screening. Applied Biochemistry and Biotechnology. 2016;179(2):283-293.
BibTex	@article{ author = {Kianmehr A and Mahrooz A and Ansari J and Oladnabi M and Shahbazmohammadi H}, title = {The Rapid and Sensitive Quantitative Determination of Galactose by Combined Enzymatic and Colorimetric Method: Application in Neonatal Screening}, journal = {Applied Biochemistry and Biotechnology}, volume = {179}, number = {2}, pages = {283-293}, year = {2016} }
RIS	TY - JOUR AU - Kianmehr A AU - Mahrooz A AU - Ansari J AU - Oladnabi M AU - Shahbazmohammadi H TI - The Rapid and Sensitive Quantitative Determination of Galactose by Combined Enzymatic and Colorimetric Method: Application in Neonatal Screening JO - Applied Biochemistry and Biotechnology VL - 179 IS - 2 SP - 283 EP - 293 PY - 2016 ER -

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