Tehran University of Medical Sciences

Science Communicator Platform

Stay connected! Follow us on X network (Twitter):
Share this content! On (X network) By
Investigating the Presence of Haemophilus Influenzae in Patients With Chronic Obstructive Pulmonary Disease (Copd) Using Molecular Method Publisher



Mohagheghmortazavi H1 ; Bahrami N2 ; Naji T3 ; Mohamadnia A4
Authors
Show Affiliations
Authors Affiliations
  1. 1. Department of Cellular and Molecular Biology, School of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  2. 2. Craniomaxillofacial Research Center, Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Basic Sciences, School of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  4. 4. Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Source: Journal of Isfahan Medical School Published:2019


Abstract

Background: Chronic obstructive pulmonary disease (COPD) is a type of obstructive pulmonary disease characterized by chronic airway blockage. The aim of this study was to investigate the presence of Haemophilus influenzae in patients with COPD using molecular method. Methods: A total of 100 samples of respiratory secretion were collected from patients with COPD. The molecular identification of Haemophilus influenza was performed using specific primers against standard kits by polymerase chain reaction (PCR) method. Findings: Haemophilus influenzae bacteria were positive in 10 out of 100 patients, and 8 of 100 were positive in PCR using specific primers. Conclusion: Nucleic acid-like PCR tests, due to their sensitivity, high accuracy, and time saving, are the appropriate method for diagnosis of Haemophilus influenzae in patients with chronic pulmonary obstruction. © 2019 Isfahan University of Medical Sciences(IUMS). All rights reserved.
Related Docs
1. The Efficiency of the Polymerase Chain Reaction Methods for the Detection of Common Pathogens in Patients With Chronic Obstructive Pulmonary Disease, Minerva Pneumologica (2020)
2. Molecular Detection of Bacterial Pathogens in Intensification of Chronic Obstructive Pulmonary Disease by Polymerase Chain Reaction Methods, Minerva Pneumologica (2019)
3. Telomere Shortening in Blood Leukocytes of Patients With Chronic Obstructive Pulmonary Disease, Tanaffos (2015)
4. Worldwide Prevalence of Viral Infection in Aecopd Patients: A Meta-Analysis, Microbial Pathogenesis (2017)
5. Molecular Detection of Chlamydia Pneumoniae in Patients With Chronic Obstructive Pulmonary Diseases With Both Real Time Polymerase Chain Reaction and Polymerase Chain Reaction Methods, Minerva Pneumologica (2019)
Experts (# of related papers)
View all Related Experts
Naghmeh Bahrami (3)
Seyed Alireza Parhiz (1)