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The Effects of Nobiletin, Hesperetin, and Letrozole in a Combination on the Activity and Expression of Aromatase in Breast Cancer Cells Publisher Pubmed



Rahideh ST1 ; Keramatipour M2 ; Nourbakhsh M3 ; Koohdani F4 ; Hoseini M5 ; Shidfar F1
Authors
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Authors Affiliations
  1. 1. Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Department of Biochemistry, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Molecular and Cellular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Cellular and Molecular Biology Published:2017


Abstract

Nobiletin (NOB) and hesperetin (HES) are the citrus polymethoxyflavone and flavonone. Aromatase or cytochrome P450 (CYP19) enzyme is a key enzyme in estrogen biosynthesis. The objective of this study was to investigate the combinational effects of HES, NOB and letrozole (LET) as aromatase inhibitors on the activity and expression of aromatase in MCF-7 cells. In this study, aromatase enzyme activity based on the conversion of androgen substrate testosterone to 17β-Estradiol was determined. Estradiol concentrations were measured using an electrochemiluminescence immunoassay. CYP19 gene expression was determined by quantitative real-time PCR. Our findings demonstrated that none of combinations including LET+NOB, LET+HES, LET+NOB+HES, and NOB+HES had no significant effects on aromatase activity and expression. The present study showed for the first time that the combination of HES, NOB, and LET had no effects on activity and expression of aromatase in MCF-7 breast cancer cells. © 2017 by the C.M.B. Association.
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1. Comparison of the Effects of Nobiletin and Letrozole on the Activity and Expression of Aromatase in the Mcf-7 Breast Cancer Cell Line, Biochemistry and Cell Biology (2017)
2. The Individual or Combinational Effects of Hesperetin and Letrozole on the Activity and Expression of Aromatase in Mcf-7 Cells, Cellular and Molecular Biology (2016)
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