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A Comparison Between the Effects of Albendazole and Meben Dazole on the Enzymatic Activity of Excretory/Secretory Prod-Ucts of Echinococcus Granulosus Protoscoleces in Vitro



Adnani Sadati SJ1 ; Farahnak A1 ; Molaei Rad MB1 ; Golestani A2 ; Eshraghiyan MR3
Authors
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Authors Affiliations
  1. 1. Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  2. 2. Dept. of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Dept. of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Source: Iranian Journal of Public Health Published:2016

Abstract

Background: Hydatid cysts are formed in human body can be treated clinically by surgery or drugs such as albendazole (ABZ) and mebendazole (MBZ). The purpose of this study was comparing the effects of ABZ and MBZ on glutathione-S-transferase, alkaline phosphatase and protease enzymes activities in protoscoleces of hydatid cyst. Methods: The culture supernatants containing the parasite Excretory/Secretory (E/S) products were collected every 12 h for 72 h. The E/S products of treated samples with 1μg/ml ABZ and MBZ and the control one were collected and after centrifugation then protein concentrations were measured according to Bradford method. GST, ALP and protease activities of E/S products were assessed photometrically. Results: The mean of GST specific activity level in treated protoscoleces with ABZ and MBZ and in control group were obtained 69.44, 132.83 and 225.47U/mg/protein/ml respectively. The mean ALP activity level in treated protoscoleces with ABZ and MBZ and in control group were detected 19.22, 22.27 and 27.85 U/mg/protein/ml respectively. The protease activity level in treated protoscoleces with ABZ and MBZ were not detected. While the mean of pro-tease activity level in control group was 7.61U/mg/proteins. Statistical analysis showed the significant difference be-tween protein concentrations, the specific activities of GST, ALP and protease enzymes in treated protoscoleces in comparison with control group (P<0.05). Also, the significant difference were seen between specific activities of GST and ALP enzymes in treated protoscoleces with ABZ in comparison with treated group with MBZ (P<0.05). Conclusion: ABZ is more effective on the enzymes activities (GST and ALP) as compared with MBZ. © 2016. Iranian Journal of Public Health. All Right Reserved.
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