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Detection of Staphylococcus Enterotoxin B (Seb) Using an Immunochromatographic Test Strip Publisher



Gholamzad M1 ; Khatami MR2 ; Ghassemi S3 ; Malekshahi ZV4 ; Shooshtari MB2
Authors
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Authors Affiliations
  1. 1. Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, IR, Iran
  2. 2. Biotechnology Research Center, Science and Technology Institute, Tehran, IR, Iran
  3. 3. Department of Pilot Nanobiotechnology, Pasteur Institute of Iran, Tehran, IR, Iran
  4. 4. Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Tehran University of Medical Sciences, Tehran, IR, Iran

Source: Jundishapur Journal of Microbiology Published:2015


Abstract

Background: Staphylococcus aureus is one of the most important microorganisms that causes various human diseases by secreting virulence factors known as staphylococcal super antigens (SAgs). Staphylococcal Enterotoxin B (SEB) is a bacterial antigen that is responsible for food poisoning in humans. Among SEB detection methods, a lateral flow device (LFD) is ideal for rapid immunochromatographic tests because it is easy to use, requires minimal time to produce results, and does not require personnel training. Objectives: In our laboratory, the production of an immunochromatographic test strip, for the detection of SEB using a sandwich assay and a competitive method, was described; the test can detect SEB with high sensitivity. Materials and Methods: The strip assays were compared with PCR, a valid method for detection. For PCR, a specific sequence for SEB production was detected using primers designed according to GenBank sequences. Results: In total, 80 food samples suspected of SEB contamination were assessed using the two methods. Fifty-four samples were contaminated based on the PCR technique and twenty-six of those were confirmed using the strip assay. Conclusions: The sensitivity of the sandwich method was approximately 10 ng/mL and that of the competitive method was approximately 250 ng/mL. In the LFD, a highly specific monoclonal antibody used for both the sandwich and competitive methods resulted in an increased sensitivity and accuracy for the detection of a minimal SEB concentration. © 2015, Ahvaz Jundishapur University of Medical Sciences.