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Intra-Arterial Drug Delivery to the Ischemic Brain in Rat Middle Cerebral Artery Occlusion Model Publisher



Azedi F1 ; Mehrpour M2 ; Kazemnejad S3 ; Mousavizadeh K4 ; Zarnani AH5, 6 ; Joghataei MT1, 7
Authors
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Authors Affiliations
  1. 1. Department of Neuroscience Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  2. 2. Department of Neurology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
  3. 3. Reproductive Biotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
  4. 4. Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran
  5. 5. Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
  7. 7. Cellular and molecular research center, Iran University of Medical Sciences, Tehran, Iran

Source: Bio-protocol Published:2019


Abstract

Rat transient middle cerebral artery occlusion (tMCAO) model is one of the most commonly used animal models in ischemic stroke studies. In the model, increasing safety and efficacy of therapeutic agent administration, such as stem cells and drugs directly to the ischemic brain using the internal carotid artery (ICA) is essential, because using the common carotid artery (CCA) for injection can close CCA completely and cause many complications after tMCAO surgery. Also, the pterygopalatine artery (PPA) is an arterial branch of the ICA that supplies blood circulation of the external part of the brain and removing the blood circulation of the PPA is required for more complete induction of ischemia to the brain. Herein, we present the insertion of intra-arterial catheter in the ICA via the external carotid artery (ECA) after the PPA in rats subjected to tMCAO surgery. Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.
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