Study of the Relationship Between Endothelial Lipase Gene Polymorphism and Serum Levels of Hdl-C, Apo A-I and Severity of Stenosis in Non-Diabetic Coronary Artery Disease Patients

Study of the Relationship Between Endothelial Lipase Gene Polymorphism and Serum Levels of Hdl-C, Apo A-I and Severity of Stenosis in Non-Diabetic Coronary Artery Disease Patients Publisher

Afshoon Z¹ ; Bahiraee A² ; Esmaeili F³ ; Mansouri E¹ ; Emami MA⁴ ; Montaseri M⁵ ; Davoodian N¹ ; Ebrahimi R³ ; Eftekhar E⁶

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1. Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
2. Department of Medical Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
3. Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
4. Cardiovascular Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
5. School of Health, Kermanshah University of Medical Sciences, Kermanshah, Iran
6. Endocrinology and Metabolism Research Center, Hormozgan University of Medical Sciences, Bandar Abbas, Iran

Source: Gene Reports Published:2020

Abstract

Background: The endothelial lipase (EL) gene is one of the most important genes in the metabolism of high-density lipoprotein cholesterol (HDL-C), and then it may be involved in the pathogenesis of coronary artery disease (CAD). The present study aimed to reveal the potential relationship between the EL 584C/T gene polymorphism and CAD severity, serum apolipoprotein A-I (Apo A-I), Apo B, and HDL-C levels in non-diabetic CAD patients. Methods: 248 subjects including 89 non-CAD controls and 159 non-diabetic CAD patients were included in the study. Blood sample was collected and used for genomic DNA extraction. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was carried out for genotyping of EL 584C/T. Serum levels of Apo A-I and Apo B were determined using the immunoturbidometric assay. The severity of coronary stenosis was determined using the Gensini score (GS). Results: The frequency of CC genotype was 52.9% and the frequency of CT and TT genotypes was 47.1% in non-CAD group. These frequencies were 59.6% and 40.4% in CAD group, respectively. There was no significant difference in the frequency of 584C/T polymorphism between CAD and non-CAD group. Moreover, the genotype and allele frequencies of the 584C/T polymorphism were not statistically different in subjects with different degrees of coronary stenosis. The serum level of Apo A-I was markedly increased in non-CAD group rather than CAD group. Those subjects with TT and CT genotypes had significantly higher levels of Apo A-I compared to those with CC genotype. Conclusion: Our results showed that 584C/T polymorphism of the EL gene was not associated with the risk of CAD. However, subjects with TT and CT genotypes showed higher Apo A-I levels compared to subjects with CC genotype suggesting a possible role of these genotype in the pathogenesis of CAD. © 2020 Elsevier Inc.

Style	Citing Format
MLA	Afshoon Z, et al.. "Study of the Relationship Between Endothelial Lipase Gene Polymorphism and Serum Levels of Hdl-C, Apo A-I and Severity of Stenosis in Non-Diabetic Coronary Artery Disease Patients." Gene Reports, vol. 21, no. , 2020, pp. -.
APA	Afshoon Z, Bahiraee A, Esmaeili F, Mansouri E, Emami MA, Montaseri M, Davoodian N, Ebrahimi R, Eftekhar E (2020). Study of the Relationship Between Endothelial Lipase Gene Polymorphism and Serum Levels of Hdl-C, Apo A-I and Severity of Stenosis in Non-Diabetic Coronary Artery Disease Patients. Gene Reports, 21(), -.
Chicago	Afshoon Z, Bahiraee A, Esmaeili F, Mansouri E, Emami MA, Montaseri M, Davoodian N, Ebrahimi R, Eftekhar E. "Study of the Relationship Between Endothelial Lipase Gene Polymorphism and Serum Levels of Hdl-C, Apo A-I and Severity of Stenosis in Non-Diabetic Coronary Artery Disease Patients." Gene Reports 21, no. (2020): -.
Harvard	Afshoon Z et al. (2020) 'Study of the Relationship Between Endothelial Lipase Gene Polymorphism and Serum Levels of Hdl-C, Apo A-I and Severity of Stenosis in Non-Diabetic Coronary Artery Disease Patients', Gene Reports, 21(), pp. -.
Vancouver	Afshoon Z, Bahiraee A, Esmaeili F, Mansouri E, Emami MA, Montaseri M, et al.. Study of the Relationship Between Endothelial Lipase Gene Polymorphism and Serum Levels of Hdl-C, Apo A-I and Severity of Stenosis in Non-Diabetic Coronary Artery Disease Patients. Gene Reports. 2020;21():-.
BibTex	@article{ author = {Afshoon Z and Bahiraee A and Esmaeili F and Mansouri E and Emami MA and Montaseri M and Davoodian N and Ebrahimi R and Eftekhar E}, title = {Study of the Relationship Between Endothelial Lipase Gene Polymorphism and Serum Levels of Hdl-C, Apo A-I and Severity of Stenosis in Non-Diabetic Coronary Artery Disease Patients}, journal = {Gene Reports}, volume = {21}, number = {}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Afshoon Z AU - Bahiraee A AU - Esmaeili F AU - Mansouri E AU - Emami MA AU - Montaseri M AU - Davoodian N AU - Ebrahimi R AU - Eftekhar E TI - Study of the Relationship Between Endothelial Lipase Gene Polymorphism and Serum Levels of Hdl-C, Apo A-I and Severity of Stenosis in Non-Diabetic Coronary Artery Disease Patients JO - Gene Reports VL - 21 IS - SP - EP - PY - 2020 ER -

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