Typing of Coa Gene Polymorphism Coding Coagulase in Staphylococcus Aureus Isolated From Foodstuffs by Pcr and Correlation to Antibiotic Resistance



Hosseini SM¹ ; Kazemian H² ; Mahmoudi H¹ ; Arabestani MR¹ Show Affiliations
Source: Journal of Chemical and Pharmaceutical Sciences Published:2016

Abstract

Background: Staphylococcus aureus could be considered a major cause of community acquired infections. Food borne diseases are important problem around the world. Coagulase is expressed in all strains of this bacterium. The main objective of this study was to determine PCR-RFLP coa patterns and relationship between coa gene and antibiotic resistances in food samples. Materials and Methods: 1050 food samples were collected during 8 months in Hamadan, Iran. Food samples were studied for S.aureus. The antibiotic susceptibility testing was performed by disk diffusion agar. After extraction of genomic DNA, nuc and coa genes were detected. Finally, with PCR-RFLP method coa typing was performed. All statistical analyses were performed using SPSS, version 22 Results: Ninety eight cases (9.33%) of S. aureus were isolated. The most frequent resistance was observed to tetracycline (41.8%), erythromycin (38.8%) and gentamicin (36.7%), respectively. Coa gene was present in all isolates and had four different patterns. Digestion of the PCR products generated four different restriction patterns for all isolates. The most frequent sizes were 700 bp and 600 bp, respectively Conclusion: In according to the increased resistance to antibiotics in strains isolated from food samples, rapid and accurate typing of S. aureus to identify the transmission of infectious agents is very important. This data can be used to better develop control measures for foods contaminated with S. aureus origin. © 2016, SPB Pharma Society. All rights reserved.