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Rapid Molecular Approach for Simultaneous Detection of Salmonella Spp., Shigella Spp., and Vibrio Cholera Publisher



Ranjbar R1 ; Naghoni A1 ; Afshar D2 ; Nikkhahi F3 ; Mohammadi M4
Authors
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Authors Affiliations
  1. 1. Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  2. 2. Department of Microbiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
  3. 3. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  4. 4. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran

Source: Osong Public Health and Research Perspectives Published:2016


Abstract

Objectives Gastrointestinal tract infection is still one of the serious public health problems in many geographic areas and is endemic in most countries including Iran. Early detection of the gastrointestinal tract pathogens can be extremely important. The aim of the current study was to apply a shortened time-multiplex polymerase chain reaction (PCR) for rapid and simultaneous detection of Salmonella spp., Shigella spp., and Vibrio cholera. Methods The standard and clinical strains of Salmonella spp., Shigella spp., and V. cholerae were used in the assay. Multiplex PCR was performed and optimized based on amplification of invA, putative integrase, and ompW genes for detecting Salmonella spp., Shigella spp., and V. cholerae, respectively. The specificity of the assay was evaluated by testing 12 different bacterial species. Results Only Salmonella spp., Shigella spp., and V. cholerae strains had positive results when subjected to the assay using multiplex PCR. The assay showed a high sensitivity, and no amplification products were observed in multiplex PCR with any of the other microorganisms. Conclusion Our study indicated that the invA, putative integrase, and ompW-based multiplex PCR assay appears to be an efficient method for rapid and simultaneous detection of Salmonella spp., Shigella spp., and V. cholerae. © 2016
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