The Effect of Royal Jelly and Propolis Alone and in Combination on Inhibition of Aspergillus Parasiticus Growth, Aflatoxin Production, and Aflr Gene Expression

The Effect of Royal Jelly and Propolis Alone and in Combination on Inhibition of Aspergillus Parasiticus Growth, Aflatoxin Production, and Aflr Gene Expression Publisher

Hamzeh Pour S¹ ; Khodavaisy S^{2, 3} ; Mahmoudi S⁴ ; Vaziri S⁵ ; Soltan Dallal MM¹ ; Oliya S⁶ ; Getso M^{3, 7} ; Rezaie S³

Show Affiliations

1. Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2. Zoonoses Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran
3. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
4. Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
5. Department of Biology, Payame Noor University, Tehran, PO Box 19395-4697, Iran
6. Department of Nutrition, School of Public Health, Qazvin University of Medical Sciences, Qazvin, Iran
7. Department of Medical Microbiology and Parasitology, College of Health Sciences, Bayero University, Kano, Nigeria

Source: Journal of Food Safety Published:2020

Abstract

The objective of this study was to determine the inhibitory effect of royal jelly (RJ) and propolis on growth, aflatoxin production and aflR gene expression in Aspergillus parasiticus. Inhibitory effect of RJ and propolis against a standard strain of A. parasiticus(ATCC 15517) was determined alone and in combination in accordance with the CLSI M38-A2 and checkerboard methods, respectively. The aflatoxin concentrations in the control and treated media were determined by HPLC. Also, the quantitative changes in the aflR gene expression were analyzed. The minimum inhibitory concentrations (MIC) of RJ and propolis alone were 3,200 and 100μg/ml, respectively. Also, the MICs of RJ and propolis in combination were 200 and 25μg/ml, respectively. When combined, a synergistic interaction was observed with a FICI of 0.312. Total levels of aflatoxin decreased from 386.1ppm to 8.72, 3.01 and 1.75ppm at 1,600μg/ml of RJ, 50μg/ml of propolis and 100+12.5μg/ml of RJ and propolis, respectively. In addition, the level of afIR gene expression was significantly decreased after treatment with RJ and propolis extracts alone and with their combination. The findings reveal that RJ and propolis extracts, either alone or in combination, have a significant inhibitory effect on aflR gene expression in aflatoxin production. © 2020 Wiley Periodicals LLC.

Related Docs

1. The Effect of Propolis on Inhibition of Aspergillus Parasiticus Growth, Aflatoxin Production and Expression of Aflatoxin Biosynthesis Pathway Genes, Journal of Environmental Health Science and Engineering (2020)

2. Aflatoxin M1 Contamination Level in Iranian Milk and Dairy Products: A Systematic Review and Meta-Analysis, World Mycotoxin Journal (2020)

3. The Inhibitory Effect of Lactic Acid Bacteria on Aflatoxin Production and Expression of Aflr Gene in Aspergillus Parasiticus, Journal of Food Safety (2018)

4. Effects of Pistacia Atlantica Subsp. Kurdica on Growth and Aflatoxin Production by Aspergillus Parasiticus, Jundishapur Journal of Microbiology (2016)

Experts (# of related papers)

View all Related Experts

Sassan Rezaie (3)

Sadegh Khodavaisy (2)

Style	Citing Format
MLA	Hamzeh Pour S, et al.. "The Effect of Royal Jelly and Propolis Alone and in Combination on Inhibition of Aspergillus Parasiticus Growth, Aflatoxin Production, and Aflr Gene Expression." Journal of Food Safety, vol. 40, no. 4, 2020, pp. -.
APA	Hamzeh Pour S, Khodavaisy S, Mahmoudi S, Vaziri S, Soltan Dallal MM, Oliya S, Getso M, Rezaie S (2020). The Effect of Royal Jelly and Propolis Alone and in Combination on Inhibition of Aspergillus Parasiticus Growth, Aflatoxin Production, and Aflr Gene Expression. Journal of Food Safety, 40(4), -.
Chicago	Hamzeh Pour S, Khodavaisy S, Mahmoudi S, Vaziri S, Soltan Dallal MM, Oliya S, Getso M, Rezaie S. "The Effect of Royal Jelly and Propolis Alone and in Combination on Inhibition of Aspergillus Parasiticus Growth, Aflatoxin Production, and Aflr Gene Expression." Journal of Food Safety 40, no. 4 (2020): -.
Harvard	Hamzeh Pour S et al. (2020) 'The Effect of Royal Jelly and Propolis Alone and in Combination on Inhibition of Aspergillus Parasiticus Growth, Aflatoxin Production, and Aflr Gene Expression', Journal of Food Safety, 40(4), pp. -.
Vancouver	Hamzeh Pour S, Khodavaisy S, Mahmoudi S, Vaziri S, Soltan Dallal MM, Oliya S, et al.. The Effect of Royal Jelly and Propolis Alone and in Combination on Inhibition of Aspergillus Parasiticus Growth, Aflatoxin Production, and Aflr Gene Expression. Journal of Food Safety. 2020;40(4):-.
BibTex	@article{ author = {Hamzeh Pour S and Khodavaisy S and Mahmoudi S and Vaziri S and Soltan Dallal MM and Oliya S and Getso M and Rezaie S}, title = {The Effect of Royal Jelly and Propolis Alone and in Combination on Inhibition of Aspergillus Parasiticus Growth, Aflatoxin Production, and Aflr Gene Expression}, journal = {Journal of Food Safety}, volume = {40}, number = {4}, pages = {-}, year = {2020} }
RIS	TY - JOUR AU - Hamzeh Pour S AU - Khodavaisy S AU - Mahmoudi S AU - Vaziri S AU - Soltan Dallal MM AU - Oliya S AU - Getso M AU - Rezaie S TI - The Effect of Royal Jelly and Propolis Alone and in Combination on Inhibition of Aspergillus Parasiticus Growth, Aflatoxin Production, and Aflr Gene Expression JO - Journal of Food Safety VL - 40 IS - 4 SP - EP - PY - 2020 ER -

Science Communicator Platform

Authors

Authors Affiliations

Abstract