Occurrence of High Resistance to Ddt in the Field Population of Arboviruses Vector Culex Pipiens Complex in Iran Publisher



Nezhad RZ¹ ; Vatandoost H^{1, 2} ; Abai MR¹ ; Djadid ND³ ; Raz A³ ; Sedaghat MM¹ ; Raeisi A⁴ Show Affiliations
Source: Asian Pacific Journal of Tropical Disease Published:2017

Abstract

Objective: To determine the susceptibility status of Culex pipiens complex to DDT. Methods: Larvae of species were collected from different breeding places and then transferred to the insectary, reared at optimal condition and then F1 from the larvae were tested against DDT. Insecticide impregnated paper of DDT 4% was provided by the Ministry of Health and Medical Education of Iran, which was purchased from the World Health Organization (WHO). Susceptibility tests were carried out according the guideline of WHO. Female mosquitoes were exposed to different interval times of imagicide and then Probit mortality regression line was plotted. From that LT50 and LT90 values were calculated. Results: The results of test indicated the higher resistance of Culex pipiens against insecticide. LT50 and LT90 values were determined as 78.39 and 305.24 h, respectively. According to the criteria of WHO, this species is resistant to DDT. Conclusions: Accurately monitoring resistance status is essential to guide the rational use of insecticides. High resistant to DDT may be due to extensive use of pesticides for pest control in home and agriculture and may confer cross resistance to other insecticides. Further studies are required to determine the mechanisms of resistance using molecular and biochemical methods. © 2017 by the Asian Pacific Journal of Tropical Disease. All rights reserved.