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Assessment of 16Srrna Methylase Genes Among Non-Esbl and Esbl-Producing Klebsiella Pneumoniae Isolates Publisher



Pakzad I1 ; Samadi N1 ; Imanieini M2 ; Taherikalani M3 ; Rahbar M4 ; Heidari R5 ; Hosainzadegan H6
Authors
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Authors Affiliations
  1. 1. Ilam University of Medical Sciences, Ilam, Iran
  2. 2. Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Lorestan University of Medical Sciences, Khorramabad, Iran
  4. 4. Department of Microbiology, Iranian Reference Health Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran
  5. 5. Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Maragheh University of Medical Sciences, Maragheh, Iran

Source: Archives of Clinical Infectious Diseases Published:2019


Abstract

The aim of our study was to investigate mechanisms of aminoglycoside resistance in extended-spectrum beta-lactamases (ESBL)-producing Klebsiella pneumoniae (K. pneumoniae) isolates from Iran. To this end, 154 clinical isolates of K. pneumoniae were collected from two hospitals in Ilam, city Iran. The Kirby-Bauer (agar diffusion) antibiotic testing method was used to determine the susceptibility pattern of the isolates against kanamycin, gentamicin, tobramycin, netilmicin and amikacin. Aminoglycoside acetyltrans-ferases (aac(3)-IIa, aac(6’)-Ib, and aac(3)-Ia), 16SrRNA methylase genes (armA and rmtB) and ESBL genes (blaTEM, blaSHV, and blaCTX-M) were detected by PCR amplification. 59.1% (n = 91) of K. pneumoniae isolates were detected ESBL producers with the phenotypic test. Moreover, blaTEM, blaSHV and blaCTX-M were detected in 83.5% (n=76), 52.7% (n=48) and 26.4% (n=24) of the ESBL-producing isolates, respectively. Among 52 resistant or intermediate isolates against aminoglycosides, the aac(3)-IIa, aac(6’)-Ib and rmtB genes were detected in 55.8% (n = 29), 80.8% (n = 42) and 1.9% (n = 1) of the isolates, respectively; none of the isolates, however, had the aac(3)-Ia and armA genes. Therefore, the results showed the high prevalence of aminoglycosides resistance in the K. pneumoniae isolates. As observed, the acetyltransferase modifying enzymes (aac genes) played major roles in determining this resistance. However, the rate of 16srRNA methylase genes was extremely low in K. pneumoniae. © 2020, Archives of Clinical Infectious Diseases.
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