Persian Gulf Jellyfish (Cassiopea Andromeda) Venom Fractions Induce Selective Injury and Cytochrome C Release in Mitochondria Obtained From Breast Adenocarcinoma Patients Publisher Pubmed



Mirshamsi MR¹ ; Omranipour R² ; Vazirizadeh A³ ; Fakhri A¹ ; Zangeneh F¹ ; Mohebbi GH⁴ ; Seyedian R⁵ ; Pourahmad J¹ Show Affiliations
Source: Asian Pacific Journal of Cancer Prevention Published:2017

Abstract

Objective: This study was conducted to investigate whether fractions of jellyfish Cassiope andromeda venom, could selectively induce toxicity on mitochondria isolated from cancer tissue of patients with breast adenocarcinomas. Methods: Firstly, we extracted two fractions, (f1 and f2) from crude jellyfish venom by gel filtration on Sephadex G-200.Then different dilutions of these extracted fractions were applied to mitochondria isolated from human breast tumoral- and extra-tumoral tissues. Parameters of mitochondrial toxicity including generation of reactive oxygen species (ROS), mitochondrial membrane potential (MMP) collapse, swelling, cytochrome c release, activation of caspase3 and apoptosis were then assayed. Result: Our results demonstrate that fraction 2 of Cassiopea andromeda crude venom significantly (P < 0.05) decreased mitochondrial succinate dehydrogenase activity, increased mitochondrial ROS production, induced mitochondrial swelling, MMP collapse and cytochrome c release, activated caspase3 and induced apoptosis only in tumoral mitochondria, and not in mitochondria obtained from extra-tumoral tissue (P < 0.05). Conclusion: In conclusion this study suggested that fraction 2 of Cassiopea andromeda crude venom selectively induces ROS mediated cytotoxicity by directly targeting mitochondria isolated from human breast tumor tissue.