Systemic Infection With Candida Albicans in Breast Tumor Bearing Mice: Cytokines Dysregulation and Induction of Regulatory T Cells

Systemic Infection With Candida Albicans in Breast Tumor Bearing Mice: Cytokines Dysregulation and Induction of Regulatory T Cells Publisher Pubmed

Ahmadi N^{1, 2} ; Ahmadi A^{1, 3} ; Kheirali E¹ ; Hossein Yadegari M⁴ ; Bayat M⁵ ; Shajiei A⁶ ; Amini AA⁷ ; Ashrafi S⁸ ; Abolhassani M¹ ; Faezi S⁹ ; Yazdanparast SA¹⁰ ; Mahdavi M^{1, 11}

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1. Department of Immunology, Pasteur Institute of Iran, Tehran, Iran
2. Department of Microbiology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
3. Department of Veterinary Medicine, Islamic Azad University, Karaj, Iran
4. Department of Mycology, Faculty of Medicine, Tarbiat Modares University, Tehran, Iran
5. Department of Microbiology, Faculty of Veterinary Specialized Sciences, Islamic Azad University Science and Research Branch, Tehran, Iran
6. Immunology Lab, Bu Ali Research Institute, Mashhad University of Medical sciences, Mashhad, Iran
7. Department of Immunology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran
8. Department of Molecular and Cellular Sciences, Faculty of Advanced Sciences and Technology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
9. Medical Biotechnology Research Center, Paramedicine Faculty, Guilan University of Medical Sciences, Rasht, Iran
10. Department of Medical Parasitology and Mycology, School of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran
11. Recombinant Vaccine Research Center, Tehran university of Medical Sciences, Tehran, Iran

Source: Journal de Mycologie Medicale Published:2019

Abstract

Objective: The effect of candidemia on immunologic parameters in breast tumor bearing patients is not well studied. Here, we hypothesised that candidemia in the tumor background may change the outcome of immunologic parameters and tumor condition. Method: Mice were divided into four groups, including normal, tumor, Candida infected (only Candidiasis) and tumor/Candidiasis groups. Tumor changes were recorded daily after tumor transplantation and induction of candidemia. Splenocytes of mice were harvested, cultured, and stimulated with PHA; afterwards, IL-4, IL-10, IFN-γ, TNF-α and TGF-β cytokines were assessed using ELISA kits. We also evaluated the population of CD4 + CD25 + Foxp3 + regulatory T cells in the tumor infiltrated and splenocytes. Results: The results showed that infection with C. albicans decreased the IFN-γ/IL-4 ratio in tumor/candidiasis and candidiasis groups versus their non-infected controls. IL-10, TGF-β and TNF-α levels increased in the candidiasis group. In addition, Candidemia led to an increase in the Treg population in tumor microenvironment and splenocytes of experimental groups compared with non-infected controls. Finally, candidemia increased tumor growth of tumor/Candidiasis group compared with the tumor group. Conclusion: It seems that systemic infection with C. albicans could not only induce regulatory T cells but also result in dysregulation of cytokine network and thereby facilitate tumor growth. © 2018 Elsevier Masson SAS

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Style	Citing Format
MLA	Ahmadi N, et al.. "Systemic Infection With Candida Albicans in Breast Tumor Bearing Mice: Cytokines Dysregulation and Induction of Regulatory T Cells." Journal de Mycologie Medicale, vol. 29, no. 1, 2019, pp. 49-55.
APA	Ahmadi N, Ahmadi A, Kheirali E, Hossein Yadegari M, Bayat M, Shajiei A, Amini AA, Ashrafi S, Abolhassani M, Faezi S, Yazdanparast SA, Mahdavi M (2019). Systemic Infection With Candida Albicans in Breast Tumor Bearing Mice: Cytokines Dysregulation and Induction of Regulatory T Cells. Journal de Mycologie Medicale, 29(1), 49-55.
Chicago	Ahmadi N, Ahmadi A, Kheirali E, Hossein Yadegari M, Bayat M, Shajiei A, Amini AA, et al.. "Systemic Infection With Candida Albicans in Breast Tumor Bearing Mice: Cytokines Dysregulation and Induction of Regulatory T Cells." Journal de Mycologie Medicale 29, no. 1 (2019): 49-55.
Harvard	Ahmadi N et al. (2019) 'Systemic Infection With Candida Albicans in Breast Tumor Bearing Mice: Cytokines Dysregulation and Induction of Regulatory T Cells', Journal de Mycologie Medicale, 29(1), pp. 49-55.
Vancouver	Ahmadi N, Ahmadi A, Kheirali E, Hossein Yadegari M, Bayat M, Shajiei A, et al.. Systemic Infection With Candida Albicans in Breast Tumor Bearing Mice: Cytokines Dysregulation and Induction of Regulatory T Cells. Journal de Mycologie Medicale. 2019;29(1):49-55.
BibTex	@article{ author = {Ahmadi N and Ahmadi A and Kheirali E and Hossein Yadegari M and Bayat M and Shajiei A and Amini AA and Ashrafi S and Abolhassani M and Faezi S and Yazdanparast SA and Mahdavi M}, title = {Systemic Infection With Candida Albicans in Breast Tumor Bearing Mice: Cytokines Dysregulation and Induction of Regulatory T Cells}, journal = {Journal de Mycologie Medicale}, volume = {29}, number = {1}, pages = {49-55}, year = {2019} }
RIS	TY - JOUR AU - Ahmadi N AU - Ahmadi A AU - Kheirali E AU - Hossein Yadegari M AU - Bayat M AU - Shajiei A AU - Amini AA AU - Ashrafi S AU - Abolhassani M AU - Faezi S AU - Yazdanparast SA AU - Mahdavi M TI - Systemic Infection With Candida Albicans in Breast Tumor Bearing Mice: Cytokines Dysregulation and Induction of Regulatory T Cells JO - Journal de Mycologie Medicale VL - 29 IS - 1 SP - 49 EP - 55 PY - 2019 ER -

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