A Convenient and Sensitive Kdna-Pcr for Screening of Leishmania Infantum Latent Infection Among Blood Donors in a Highly Endemic Focus, Northwestern Iran Publisher Pubmed



Asfaram S^{1, 2} ; Fakhar M² ; Mohebali M³ ; Ziaei Hezarjaribi H² ; Mardani A⁴ ; Ghezelbash B⁵ ; Akhoundi B³ ; Zarei Z³ ; Moazeni M⁶ Show Affiliations
Source: Acta Parasitologica Published:2022

Abstract

Background: Recent global evidences showed that asymptomatic blood donor carriers of Leishmania infection will appear as a threat for blood transfusions recipients in endemic areas. As yet, there is no appropriate diagnostic procedure for detecting infection of blood donors in blood banks. Subjects and Methods: The present study was aimed to apply various current diagnostic tests among blood donors in an endemic area of visceral leishmaniasis (VL), Ardabil Province, northwestern Iran. Blood samples were gathered from 860 blood donors in endemic areas of the province between 2017 and 2018, at eight blood donation centers. These samples was assessed using microculture, serological (DAT and rK39-ICT) and molecular based (conventional kDNA-PCR and HRM-PCR) tests. Results: Of 860 eligible donors, 24 (2.8%) were seropositive for VL by DAT, and 388 (45%) were positive by kDNA-PCR. Moreover, 19 (19/860) were positive for both of them. Out of 19 subjects, 5.3% (1/19) was positive by rK39-ICT, 10.5% (2/19), and 79% (15/19) were detected positive in microculture and HRM-PCR methods, respectively. Nineteen donors were followed up for 2 years, of which 16 (84.2%) had a serological conversion, and 4 (21%) were positive by kDNA-PCR. The sensitivity of kDNA-PCR, and HRM-PCR procedures in detecting Leishmania parasite was found to be 98.7%, and 79%, respectively. Conclusions: Our findings justify the use of kDNA-PCR as a convenient and sensitive tool for screening subjects with leishmanial latent infection in blood banks at least in endemic regions. In these areas, however, a PCR-based test should be used to validate Leishmania infection among seropositive donors. © 2022, The Author(s) under exclusive licence to Witold Stefanski Institute of Parasitology, Polish Academy of Sciences.