Upregulation of Tgf-Β Type Ii Receptor in High Glucose-Induced Vascular Smooth Muscle Cells Publisher Pubmed



Ghasempour G^{1, 4} ; Mohammadi A⁵ ; Zamanigarmsiri F⁶ ; Soleimani AA¹ ; Najafi M^{1, 2, 3} Show Affiliations
Source: Molecular Biology Reports Published:2022

Abstract

Background: Mortality in patients with diabetes mellitus is estimated above 65% due to cardiovascular diseases. The aim of study was to investigate the effects of high-glucose conditions on TGF-β type II receptor (TGFBR2) expression levels, cell viability, and migration rate in vascular smooth muscle cells (VSMCs). Methods: VSMCs were incubated in 30 mM and 50 mM of glucose for 24 h, 48 h, and 72 h periods. The gene and protein expression levels were investigated by Real-time qRT-PCR and western blotting techniques, respectively. The cell viability was evaluated by MTT assay. VSMC migration rate was also studied by wound healing assay. Results: The TGFBR2 gene and protein expression levels were significantly upregulated in all the groups treated with glucose in 24 h, 48 h, and 72 h periods. The cell viability was not significantly affected in values of 30 mM and 50 mM of glucose. The increase of migration rate of VSMCs was not significant. Conclusion: The results suggested the increased expression levels of TGFBR2 in the response to high glucose conditions may modulate the cellular events through the signaling pathway network in VSMCs. © 2021, The Author(s), under exclusive licence to Springer Nature B.V.