The Effect of Impregnated Alpha-Cellulose Nanofibers With Ciprofloxacin Hydrochloride on Staphylococcus Aureus in Vitro



Yazdanbakhsh MF¹ ; Rashidi A^{1, 2} ; Rahimi MK² ; Khajavi R³ ; Shafaroodi H⁴
Source: Bulgarian Chemical Communications Published:2018

Abstract

The main objective of this analytical and descriptive study is to evaluate the effect of alpha-cellulose nanofibers impregnated with ciprofloxacin hydrochloride on a staphylococcus aureus culture. In this regard, after conducting various studies on biocompatible and natural polymers, wheat bran was selected as a cellulosic-based biocompatible agricultural waste. After alpha-cellulose extraction from wheat barn by the ultrasonic-Soxhlet method. the alpha-cellulose investigated with FTIR. FTIR spectra showed that holocellulose was largely removed during alkali treatment under ultrasonic waves. the alpha-cellulose nanofibers were prepared by employing the electrospinning technique. from which the cellulose disks were prepared. Then, these disks and paper disks (without antibiotics) were placed in 3.3% ciprofloxacin hydrochloride. The mentioned disks were placed on the Staphylococcus aureus cultured medium alongside standard disks and the results were measured as the inhibition zone after 24 hours. Both disks (alpha-cellulose nanofibers and paper) containing ciprofloxacin hydrochloride created the inhibition zone on the S. aureus medium. The concentration of ciprofloxacin hydrochloride adsorbed by cellulose was determined by comparing the effects of cellulose disks containing different concentrations of antibiotics and standard ciprofloxacin disks. © 2018 Bulgarian Academy of Sciences, Union of Chemists in Bulgaria.