Mxenes for Crispr-Based Diagnostics: Innovations and Advancements Publisher



Amoozadeh M ; Mohandesnezhad S ; Zarepour A ; Khosravi A ; Zarrabi A ; Iravani S
Source: Microchemical Journal Published:2025

Abstract

The incorporation of MXenes into CRISPR-based diagnostics marks a transformative advancement in molecular testing across environmental monitoring, biomedical diagnostics, and food safety. MXenes, a class of two-dimensional materials characterized by their exceptional electrical conductivity, hydrophilicity, and customizable surface properties, significantly enhance the sensitivity and specificity of CRISPR assays. Recent developments demonstrate that MXene composites improve the stability and performance of CRISPR systems, facilitating rapid and accurate detection of pathogens and genetic markers. These capabilities support applications such as analyzing environmental DNA (eDNA), point-of-care testing for infectious diseases, and identifying contaminants in food products. Despite these advancements, challenges such as sample processing, stability, the necessity for nucleic acid amplification, and regulatory barriers complicate practical implementations. This review explores the recent advancements of MXene-assisted CRISPR diagnostics, examines the challenges hindering their broader application, and discusses future perspectives for improving detection capabilities and integrating innovative technologies to enhance diagnostic efficacy. © 2025 Elsevier B.V., All rights reserved.