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Leishmania Tropica Infected Human Lesions: Whole Genome Transcription Profiling Publisher Pubmed



Masoudzadeh N1 ; Mizbani A2 ; Taslimi Y1 ; Mashayekhi V3 ; Mortazavi H4 ; Sadeghipour P1 ; Ardekani HM1 ; Rafati S1
Authors

Source: Acta Tropica Published:2017


Abstract

Leishmania (L.) tropica is the main causative agent of anthroponotic cutaneous leishmaniasis (CL) in Iran. Defining the host inflammatory response in the L. tropica lesions are crucial for the development of new treatment modalities. High-throughput RNA sequencing provides a powerful method for characterization of the human gene expression profile in L. tropica lesions. Comparing the transcription profile of the L. tropica skin lesions with normal skin identified over 5000 differentially regulated genes. Gene set enrichment analysis indicated significant activation of key immunological pathways related to antigen processing and presentation. In addition, we observed a substantial upregulation of immunoglobulin genes in lesion samples, highlighting the remarkable involvement of B cells in the infection site. To our knowledge, this study is the first report to build a comprehensive picture of transcriptome changes in acute human skin lesions during infection by L. tropica. © 2017 Elsevier B.V.
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