Phyto-Fabrication of Silver Nanoparticles Using Typha Azerbaijanensis Aerial Part and Root Extracts



Mirzaie A¹ ; Badmasti F² ; Dibah H³ ; Hajrasouliha S³ ; Yousefi F³ ; Andalibi R⁴ ; Kashtali AB³ ; Rezaei AH⁵ ; Bakhtiatri R⁶ Show Affiliations
Source: Iranian Journal of Public Health Published:2022

Abstract

Background: Silver nanoparticles (AgNPs) were phyto-synthesized using Typha azerbaijanensis aerial part and root extracts, and their biological activities were investigated. Methods: This study was conducted in the Science and Research Branch, Islamic Azad University, Tehran, Iran in 2019. In this experimental study, silver nanoparticles (AgNPs) were phyto-synthesized and the physicochemi-cal properties of AgNPs were determined using UV-Vis (UV-Vis) spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier transform infrared (FTIR) spectroscopy. Antibac-terial and anticancer activity of synthesized AgNPs was determined using microdilution assay, and MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) methods, respectively. The apoptotic effects of AgNPs were investigated using Real-Time PCR and flow cytometry techniques. Results: Morphological analysis of the synthesized AgNPs confirmed the spherical shape of AgNPs with an average size of 10.67 to 16.69 nm. The FTIR spectrum confirmed the presence of phytochemicals from T. azerbayenensis extract at the AgNP surface. Antibacterial experiments showed that phyto-fabricated AgNPs had significant antibacterial activity against Gram-negative bacteria. The AgNPs were significantly cytotoxic against breast cancer cell line (MCF-7) through induction of apoptosis. Conclusion: The phyto-synthesized AgNPs had biological activities could be useful in pharmaceutical applica-tions. © 2022 Mirzaie et al. Published by Tehran University of Medical Sciences.