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Mercury (Ii) Sensing Using a Simple Turn-On Fluorescent Graphene Oxide Based Aptasensor in Serum and Water Samples Publisher Pubmed



Chaghazardi M1, 2 ; Kashanian S3, 4 ; Nazari M1 ; Omidfar K2, 5 ; Shariatirad M6 ; Joseph Y7, 8 ; Rahimi P7, 8
Authors
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Authors Affiliations
  1. 1. Faculty of Chemistry, Razi University, Kermanshah, Iran
  2. 2. Biosensor Research Center, Endocrinology and Metabolism Molecular–Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  3. 3. Faculty of Chemistry, Sensor and Biosensor Research Center (SBRC) & Nanoscience and Nanotechnology Research Center (NNRC), Razi University, Kermanshah, Iran
  4. 4. Nanobiotechnology Department, Faculty of Innovative Science and Technology, Razi University, Kermanshah, Iran
  5. 5. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran
  6. 6. Department of Analytical Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran
  7. 7. Institute of Nanoscale and Biobased Materials, Faculty of Materials Science and Materials Technology, Technische Universitat Bergakademie Freiberg, Freiberg, 09599, Germany
  8. 8. Water Research Center, Technische Universitat Bergakademie Freiberg, Freiberg, 09599, Germany

Source: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy Published:2024


Abstract

A simple, highly sensitive, and selective fluorometric aptasensing platform based on aptamer and graphene oxide (GO) is proposed for the determination of mercury (II) ion (Hg2+). In the designed assay, two aptamer probes, a carboxy-fluorescein (FAM) labeled aptamer (aptamer A) and its complementary (aptamer B) with partial complement containing several mismatches and GO as the quencher were used. In the absence of Hg2+, both A and B aptamers were adsorbed on the surface of GO by π-π-stacking, leading to fluorescence quenching of FAM due to fluorescence resonance energy transfer (FRET). Upon exposure to Hg2+, the A and B aptamer strands bind Hg2+ and form T-Hg2+-T complexes, leading to the formation of a stable double-stranded aptamer. The double-stranded aptamer is detached from the GO surface, resulting in the recovery of FAM fluorescence. The fluorescence intensity (FI) of the developed sensor was correlated with the Hg2+ concentration under optimized experimental conditions in two wide linear ranges, even in the presence of 10 divalent cations as interferences. The linear ranges were obtained from 200.0 to 900.0 fM and 5.0 to 33.0 pM, a limit of detection (LOD) of 106.0 fM, and a limit of quantification (LOQ) of 321.3 fM. The concentration of Hg2+ was determined in five real samples containing three water and two serum samples, using spiking and standard addition methods and the results were compared with the spiked amounts and atomic absorption (AAS) as standard method respectively, with acceptable recoveries. Furthermore, in the standard addition method, to overcome the effects of matrix influence of real samples in quantitative predictions, the excitation-emission matrix (EEM) data for samples was simultaneously analyzed by multivariate curve resolution with alternating least squares (MCR-ALS) as a second-order standard addition method (SOSAM). © 2024 Elsevier B.V.
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1. Fluorometric Mercury (Ii) Detection Using Heteroatom-Doped Carbon and Graphene Quantum Dots, Photonics (2024)
2. Nitrogen and Sulfur Co-Doped Carbon Quantum Dots Fluorescence Quenching Assay for Detection of Mercury (Ii), Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy (2023)
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