Exhaled Breath Condensate Efficacy to Identify Mutations in Patients With Lung Cancer: A Pilot Study

Exhaled Breath Condensate Efficacy to Identify Mutations in Patients With Lung Cancer: A Pilot Study Publisher Pubmed

Kazeminasab S¹ ; Ghanbari R² ; Emamalizadeh B³ ; Jouybangharamaleki V^{4, 5} ; Taghizadieh A⁶ ; Jouyban A^{1, 7} ; Khoubnasabjafari M^{6, 8}

Show Affiliations

1. Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran
2. Digestive Oncology Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran
3. Molecular Medicine Research Center and Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
4. Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5. Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz, Iran
6. Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
7. Faculty of Pharmacy, Near East University, Mersin 10, Cyprus
8. Department of Anesthesiology and Intensive Care, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Source: Nucleosides# Nucleotides and Nucleic Acids Published:2022

Abstract

Exhaled breath condensate (EBC) is used to investigate the efficacy of EBC to detect the genetic mutations in patients with lung cancer. Samples of 5 patients and 5 healthy volunteers were collected. DNA was extracted and used for amplification of hotspot regions of TP53 and KRAS genes by using PCR. We performed the mutation analysis by direct sequencing in all subjects. Detected mutations in EBC samples were compared with those of corresponding tumor tissues and there was complete agreement within the detected mutations in EBC and tumorous tissue. EBC can be used as an efficient and noninvasive source for the assessment of gene mutations in patients with lung cancer. © 2022 Taylor & Francis Group, LLC.

2. Microextraction and Chromatographic Analysis of Budesonide Epimers in Exhaled Breath Condensate, Current Analytical Chemistry (2020)

3. Exhaled Breath Condensate As a Potential Specimen for Diagnosing Covid-19, Bioanalysis (2020)

4. Waste of Mechanical Ventilators As a Biological Sample for Follow up of Biomarkers and Drugs, Pharmaceutical Sciences (2020)

Experts (# of related papers)

Reza Ghanbari (1)

Style	Citing Format
MLA	Kazeminasab S, et al.. "Exhaled Breath Condensate Efficacy to Identify Mutations in Patients With Lung Cancer: A Pilot Study." Nucleosides# Nucleotides and Nucleic Acids, vol. 41, no. 4, 2022, pp. 370-383.
APA	Kazeminasab S, Ghanbari R, Emamalizadeh B, Jouybangharamaleki V, Taghizadieh A, Jouyban A, Khoubnasabjafari M (2022). Exhaled Breath Condensate Efficacy to Identify Mutations in Patients With Lung Cancer: A Pilot Study. Nucleosides# Nucleotides and Nucleic Acids, 41(4), 370-383.
Chicago	Kazeminasab S, Ghanbari R, Emamalizadeh B, Jouybangharamaleki V, Taghizadieh A, Jouyban A, Khoubnasabjafari M. "Exhaled Breath Condensate Efficacy to Identify Mutations in Patients With Lung Cancer: A Pilot Study." Nucleosides# Nucleotides and Nucleic Acids 41, no. 4 (2022): 370-383.
Harvard	Kazeminasab S et al. (2022) 'Exhaled Breath Condensate Efficacy to Identify Mutations in Patients With Lung Cancer: A Pilot Study', Nucleosides# Nucleotides and Nucleic Acids, 41(4), pp. 370-383.
Vancouver	Kazeminasab S, Ghanbari R, Emamalizadeh B, Jouybangharamaleki V, Taghizadieh A, Jouyban A, et al.. Exhaled Breath Condensate Efficacy to Identify Mutations in Patients With Lung Cancer: A Pilot Study. Nucleosides# Nucleotides and Nucleic Acids. 2022;41(4):370-383.
BibTex	@article{ author = {Kazeminasab S and Ghanbari R and Emamalizadeh B and Jouybangharamaleki V and Taghizadieh A and Jouyban A and Khoubnasabjafari M}, title = {Exhaled Breath Condensate Efficacy to Identify Mutations in Patients With Lung Cancer: A Pilot Study}, journal = {Nucleosides# Nucleotides and Nucleic Acids}, volume = {41}, number = {4}, pages = {370-383}, year = {2022} }
RIS	TY - JOUR AU - Kazeminasab S AU - Ghanbari R AU - Emamalizadeh B AU - Jouybangharamaleki V AU - Taghizadieh A AU - Jouyban A AU - Khoubnasabjafari M TI - Exhaled Breath Condensate Efficacy to Identify Mutations in Patients With Lung Cancer: A Pilot Study JO - Nucleosides# Nucleotides and Nucleic Acids VL - 41 IS - 4 SP - 370 EP - 383 PY - 2022 ER -

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